(70512, Cell Signaling Technology, Danvers, MA, USA) and mouse antibodies to TWIST1 (1:100, sc-81417, Santa Cruz Biotechnology, Dallas, TX, USA); (5) rabbit antibodies to KLF2 (1:200, SL2772R, Sunlong Biotech, Hangzhou, China) and mouse antibodies to TWIST1 (1:200, sc-81417, ...
Figure 1B showed the SEM of gold nanofilm. 6. PDMS casting: The pre-polymer and the curing agent of PDMS were mixed in a 10:1 ratio by weight. After thermal coagulation, the PDMS can be obtained. 7. Bonding of the SU-8 with the PDMS: The surface-treated PDMS was tightly bound ...
Moreover, the in vivo test of the 3DP SF implants showed that the material could retain shape up to three months with minimal inflammatory response and promote tissue integration in a mouse model [116]. Due to its unparalleled advantages, 3D printing will play an important role in future ...
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After treatment with cytotoxic drugs as described above, cells from co-cultures were labelled with LIVE/DEAD Aqua stain, as well as with PE mouse anti-human CD73 (BD Pharmingen, San Jose, CA, USA) to differentiate BM-MSC from H929 cells. Co-cultures with non-treated BM-MSC were used ...