Multi-enzymeCascade reactionsBiocatalysisNanotechnologyMulti〆nzyme cascade reactions are indispensable in biotechnology and many industrial (bio)chemical processes. However, most natural enzymes have poor stability and reusability, and tend to inactivate in toxic media or high temperature, which significantly...
In this work, we reported a cholesterol oxidase (Chox)-loaded platinum (Pt) nanozyme with the collaborative cascade nanoreactor for the construction of nanozyme–enzyme-linked immunosorbent assay (N-ELSA) models to realize high-throughput rapid evaluation of cancer markers. Considering the high ...
Multi〆nzyme cascade reactions are indispensable in biotechnology and many industrial (bio)chemical processes. However, most natural enzymes have poor stability and reusability, and tend to inactivate in toxic media or high temperature, which significantly limit their broader applications. Metal﹐rganic...
Two steps in one pot: enzyme cascade for the synthesis of nor(pseudo)ephedrine from inexpensive starting materials. Angew. Chem. Int. Ed. 52, 6772–6775 (2013). Article CAS Google Scholar Sehl, T. et al. Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo)...
EnzymeSubtilisin CarlsbergCandida antarctica lipase BHIV13-OxathiolaneAn enantiopure (2R,5R)-1,3-oxathiolane was obtained using a multienzymatic cascade protocol. By employing a combination of surfactant-treated subtilisin Carlsberg and Candida antarctica lipase B, the absolute configuration of the ...
Self-Sufficient In Vitro Multi-Enzyme Cascade for Efficient Synthesis of Danshensu from l-DOPA 来自 ACS 喜欢 0 阅读量: 2 作者:Ruizhi Han,Ke Gao,Yulin Jiang,Jieyu Zhou,Guochao Xu,Jinjun Dong,Ulrich Schwaneberg,Yu Ji,Ye Ni 摘要: Danshensu (DSS), a traditional Chinese medicine, is widely ...
Scaffold protein is a new form that mimics the natural multi-enzyme complex cascade reaction. Some macromolecular protein complexes have a special protein–protein interaction. The scaffold proteins are defined as proteins that organize signal complexes by binding at least two signal enzymes together and...
TCPP and ZIF-8 were successfully combined to construct a photocatalytic multi-enzyme cascade biomimetic carbon sequestration system for the first time. TCPP was used as the photocatalyst and ZIF-8 was used as the multi-enzyme immobilized carrier for FateDH and FaldDH. The TCPP content, material ...
The amination reaction was optimized with respect to cofactor addition and enzyme dosage. Isopropanol was added to decrease ketone intermediate accumulation in the amination step, which further enhanced the overall conversion. This cascade system converted a panel of hydrocarbon substrates into the ...
The assembly of this three-enzyme cascade demonstrated not only that the methodology developed allows the coupling of three step cascade reactions, but also that the throughput for the cascade is enhanced by arranging the enzymes at the nanoscale and by increasing the local concentration of the ...