Multi-Cloning Site Multi-Cluster Interleaving Multi-Coated Multi-Code Direct Sequence Spread Spectrum Multi-Code Interference in the Frequency Domain Multi-Code Interference in the Time Domain Multi-Code Multi-Packet Transmission Multi-Code/Variable Spreading Factor ...
Optimization of codons and in silico cloning The XHOI and BamHI restriction endonuclease sites were selected and the codons of the vaccine were analyzed and optimized using the JAVA codon adaptation tool (http://www.jcat.de/). The DNA sequence of the plasmid was obtained from the Plasmid Files...
以下哪种元件不是克隆载体的质粒所必须的?A、A multicloning site 多克隆位点B、A selectable Marker 选择标记C、An Origin of Replication 复制起点D、A Promoter 启动子
A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 bp PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the reco...
The process of cloning your website is a little breeze. Once you have the URL of the new website specified, select the website you want to clone in NS Cloner’s settings, enter the desired URL, add title and you are done. One more click and let NS Cloner do the magic right before...
and GC content of 46%. The downstream primer sequence is 5’CTCGAGTGCTGCCCACTCAGACTT, with a length of 24-mer, a Tm value of 64°C, and GC content of 58%.The target gene was amplified using simulated PCR in SnapGene and inserted into the multiple cloning site (MCS) region of the pl...
Fixes an additional issue with large file transfers related to HTTP commands (e.g., Github repository cloning) where a proxy timeout would result in a 408 status code. Fixes an issue where traffic is matched to a correct policy, but an incorrect ce...
Cloning,Expression,and Characterization of the Squid Na+–Ca2+ Exchanger (NCX-SQ1) We have cloned the squid neuronal Na+–Ca2+ exchanger,NCX-SQ1,expressed it in Xenopus oocytes,and characterized its regulatory and i... Z He,Q Tong,BD Quednau,... - 《Journal of General Physiology》 被引...
As a last resort, site-specific point mutations in the toxin gene that render the toxin harmless for the cell can be introduced to enable the cloning and overexpression of the non-toxic protein. Examples of such non-toxic mutants are E. coli MazFE24A (EcMazFE24A) or bacteriophage P1 ...
In today's world of agile marketing, you cannot afford sites that take months to develop and release. Launch new content, pages or sites by simply cloning and modifying existing resources. No need to start from scratch! Utilize all existing content, templates and themes available on any of yo...