Mucinsare high-molecular-weight, heavilyglycosylated proteinsthat contribute to the gel-like qualities ofmucusand are critical for mucosal host defense. Multiple mucin genes are expressed in the airways, but the primary gene products in mucus areMUC5ACandMUC5B.53Mucins are formed in theGolgi appara...
The therapeutic tractability of these targets in the appropriate direction (downregulation of MUC5AC and TNFRSF1A, upregulation of IL6R) was then assessed. Firstly, inhibitors of MUC5AC have been in development for oncology, with evidence that compounds like flavonoids, alkaloids, and glycosides ...
different core: m/z = 407.167 indicating a HexNAc2 structure was detected in several HCD spectra representing four different proteins, ITI H4 (Q5EA67), insulin-like growth factor II (P07456), kininogen-1 isoform (F1MNV4), and hepatocyte growth factor activator (E1BCW0) (supplemental Table ...
However, glycosylation of highly expressed recombinant glycoproteins, like magnICON-expressed E. coli enterotoxin B subunit:H. sapiens mucin 1 tandem repeat-derived peptide fusion protein (LTBMUC1), is limited by the low endogenous UDP-GalNAc substrate pool and the insufficient translocation of UDP-...
Such conformational impact on the underlying peptides was proved to be remarkable in the glycosylation at the consecutive Thr residues of MUC5AC.doi:10.1002/chem.201002754Ryo HashimotoGraduate School of Life Science and Frontier Research Center for Post-Genome Science and Technology Hokkaido University ...
Importantly, the decreased basal cellular mucin content in cells with knocked- down levels of IRE1b was coupled to a decreased basal secretion of MUC5AC protein (Figure 8g,h). We evaluated the impact of knocking down IRE1a on mucin production in Calu-3 cultures stably expressing an IRE1a...
The complete type, or type I, is characterized by a clear loss of expression of the mucins MUC1, MUC5AC, and MUC6, which are normally present in the gastric mucosa, and aberrant expression of the intestinal MUC2 mucin in goblet cells (Figure 1bI). The incomplete type (including the ...