Jean-Luc Desseyn, ... Valérie Gouyer, in Gene, 2008 MUC3A, MUC3B, MUC11, MUC12 and MUC17 are organized in a cluster of genes at locus 7q22 (Fox et al., 1992; Gum et al., 2002; Pratt et al., 2000). If the full genomic and cDNA sequences have been reported to date for...
The results (mean±SD of three determinations) are expressed as relative luciferase activity as compared to that obtained for cells transfected with the pBRN2-Luc (WT) vector (assigned a value of 1). f LNCaP-AI/tet-CshRNA (left panel), LNCaP-AI/tet-MUC1shRNA (middle panel) and LNCaP...
Cells were transfected with 300 or 600 ng of the reporter plasmids containing 1745 bp of the cyclin D1 gene promoter ( À 1745CD1Luc, TOPFLASH) consists of wild-type LEF-1/Tcf-4-binding site or reporter plasmids containing mutated LEF-1/Tcf-4-binding site in the cyclin D1 gene ...
Control cells were treated with an equivalent amount of vehicle. 2.2. Plasmids pGL3-basic and pRL-null were purchased from Promega, pcDNA/TO from Invitrogen, pFLAG-CMV2 from Sigma and pBSK(-) from Stratagene. pMUCDHL 1.4 kb-Luc in pGL3-basic vector was created in our laboratory [25] and...
图4(a):对雌性NSG小鼠i.p.接种SKOV3‑MUC16‑GFP‑LUC肿瘤细胞,并且随后用ecto单独的T细胞或T细胞和MUC16‑BiTED处理,并随时间对肿瘤负荷进行成像。图4(b):来自ecto图4(a)的生物发光的定量。图4(c):用单独的T细胞或T细胞和MUC16‑BiTED处理雌性NSG...
JeanLuc DavignonBrain Research BulletinAndrea, RinerKirk, Chan-TackJeffrey, Murray. (2009) Original Research: Intravenous Ribavirin-Review of the FDA's Emergency Investigational New Drug Database (1997-2008) and Literature Review. Postgraduate Medicine 121 , 139-146 /...
enrichment compared to that obtained with the IgG control (assigned a value of 1).eLNCaP-AI cells were transfected with pGL3-Basic Luc, pBRN2-Luc (WT), pBRN2-Luc MUT1 or pBRN2-Luc MUT2 for 48 h and then analyzed for luciferase activity. The results (mean±SD of three ...
However, it is not fully curative for advanced cervical cancer due to recurrence, metastasis, or lack of an optimal treatment option11,12. Neoadjuvant chemotherapy (NACT) has been used for treatment of cervical cancer, mainly for patients with advanced cervical cancer, which is usually a ...
After thawing, cells were assayed for luciferase activity using LucLite reagent (Packard) and a TopCount luminometer (Packard). RESULTS RACE-PCR, cDNA Cloning and Sequence Determination of the MUC5AC Amino Terminus Based on the 99% sequence identity between MUC5AC clone JUL 32 (19) and HGM-...
Debat et al., “Overpassing an aberrant Vkappa gene to sequence an anti-idiotypic abzyme with beta-lactamase-like activity that could have a linkage with autoimmune diseases.” FASEB, 15:815-822 (2001). GenBank Accession No. AJ277812.1, “Mus musculus partial mRNA for immunoglobulin kappa...