以下的protocol供参考: --1. Make a solution of 5 mg/mL MTT1 dissolved in PBS and filter sterilized. --2. 5 hours before the end of the incubation add 20 uL of MTT solution from step one to each well containing cells. --3. Incubate the plate in a CO2 incubator at 37 oC for 5 ...
MTT Cell Assay ProtocolHorton, TNotes, Important AssaySpring, ColdProtoc, HarbAlerting, EmailKuhn, D MBalkis, MChandra, JMukherjee, P KGhannoum, a
MTT操作步骤(英文)
BASICPROTOCOL Ifyouarefamiliarwiththeprocedureandknowthecellcounttouseinyourspecificassay,youmayfollowthisbasicprotocol. 1Platecellsat1000to100,000perwellIncubatefor6to24hoursAdd10µlMTTReagentIncubatefor2to4hoursuntilpurpleprecipitateisvisibleAdd100µlDetergentReagentLeaveatroomtemperatureinthedarkfor2hoursReco...
This protocol should not be used for: Fluorescence microscopy You will need the following for this protocol: Cells growing in culture CyQUANT MTT Cell Viability Assay (Cat. No. V-13154) Cell culture medium Phosphate-buffered saline (PBS) (Cat. No. 10010-023) ...
Nov 07 1 Proliferation Assay MTT Protocol Ba ckground - Traditionally, the determination of cell growth is done by counting viable cells after staining with a vital dye. Several approaches have been used in the past. Trypan blue staining is a simple way to evaluate cell membrane integrity (and...
Cytotoxicity MTT Assay is a colorimetric assay which measures cell viability, proliferation and cytotoxicity.
MTT操作步骤(英文)
Our protocol has the advantage of a thorough HBSS wash step in the form of the MTT assay buffer to remove any phenol red remaining from the complete media. This wash step removes chemicals added previously to induce or prevent cellular damage, thus stopping or slowing the specific treatment, ...
This protocol should not be used for: Fluorescence microscopy You will need the following for this protocol: Cells growing in culture CyQUANT MTT Cell Viability Assay (Cat. No. V-13154) Cell culture medium Phosphate-buffered saline (PBS) (Cat. No. 10010-023) ...