摘要在抗肿瘤药物的体外筛选中,M’FF法和SRB法是常用的两种方法.我们用MTT 法和SRB溘分别测定3种巳知植物抗癌药对22株人肿瘤细胞的抗癌活性,对这两种方 法进行了详细的比较.通过分析两种方法测出的细胞存活率(T/C)的差异分布和相关系 数以及Icso的二变量分布,比较了两种方法谢定结果的异同{通过两种方法重复测定...
MTT法,即四唑盐比色法,通过检测活细胞线粒体中脱氢酶的活性来反映细胞存活情况;而SRB法,即磺酰罗丹明B法,则通过染色后测定细胞蛋白质总量来评估细胞增殖。两种方法各有特点,适用于不同阶段的药物筛选。ThisarticlewilloutlinethebasicprinciplesofMTTandSRBmethodsandtheirapplicationsinanti-tumordrugscreening.TheMTT...
Clear differences between cell survival curves obtained using the clonogenic, microtetrazoline (MTT) and sulforhodamine B (SRB) assays are shown. The most sensitive of the assays investigated was the clonogenic assay. The MTT and SRB assays were found to be relatively insensitive especially at ...
抗肿瘤药物筛选中MTT法和SRB法的比较_谭卫东.pdf, Vol. 11 No. 3 N ATURAL PRODU CT RESEARCH AND DEVE LO PM EN T 17 M TT SRB ( 610041) , M T T SRB。 M TT SRB 3 , 。 ( T /C) ,; IC50 3 7 ,; T /C,。 : M T T SRB,, SRB , 3。 M T T , SRB, ,, ,, 90。 [ ...
and estimated the bivariate distribution of the values for IC 50 of the two assays.We also observed the variation of T/C as the change of time.The results indicate that within the limits of data both assays perform similarly,either assay can be used in screening application.The SRB assay ...
SRB法与MTT法细胞计数应用比较 第１５卷第１７期２００５年９月 中国现代医学杂志 ＣｈｉｎａＪｏｕｒｎａｌｏｆＭｏｄｅｍＭｅｄｉｃｉｎｅ Ｖ０１．１５Ｎｏ．１７ Ｓｅｐ．２００５ 文章编号：１００５—８９８２（２００５）１７—２６１５－０３ ＳＲＢ法与ＭＴＴ法细胞计数应用比较 周思朗ｔ，屈艳妮ｚ，张健，，...
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Thus, the aim of this study was to evaluate the cytotoxicity of a Thai herb by comparing MTT and SRB assay results. Methods Test materials We used a powder formulation of the Thai herb Phaya-Yor in Fig. 1, also known as Clinacanthus nutans (Twin Lotus Co., Ltd., Bangkok, Thailand). ...
1)MTT colorimetric assayMTT比色分析法 1.?Method MTT colorimetric assay was used to measure the growth of MKN45 cell cultured in RPMI 1640 medium with and without 10% FBS, respectively.方法 采用MTT比色分析法分别测定OCT对生长于无血清培养基及含 1 0 %胎牛血清 (FBS)培养基中MKN45细胞的调控作...
8.Correlation between experimental results of MTT colorimetric assay and SRB assay in anti-tumor drug screening in vitroSRB法和MTT法抗肿瘤药物筛选结果相关性研究 9.These cells lay together with features of epithelial cells, forming a monolayer.MTT及细胞计数法所测细胞生长过程基本一致; 10.Detecting th...