HUVECs were seeded at a density of 1 × 105 cells/well on the bottom side of the Transwell insert (1.0 µm pore size; Becton Dickinson), which was placed in the 12-well plate overnight. The permeability was assayed by measuring the leakage of Evans blue (Sigma‒Aldrich) ...
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Tube formation assays were performed as described previously29. Briefly, 50 μL of growth factor-reduced Matrigel (Becton Dickinson) was dispensed into a 96-well plate at 4 °C. After gelling for at least 10 min at 37 °C, pretreated MCECs or HUVECs (exposed to NG or HG condi...