结论:鼻咽癌细胞中DDX6通过破坏CKMT1A mRNA稳定性,负调控CKMT1A蛋白质翻译,增强鼻咽癌细胞增殖和迁移能力,从而促进鼻咽癌恶性进展。 [关键词]鼻咽癌;CKMT1A;DDX6;细胞增殖;细胞迁移 [Abstract]Background and purpose:DDX is an adenosi...
ESCC 细胞中的免疫共沉淀 (co-IP) 实验证实 PEBP1 和 MTHFD2 相互结合(图 5D 和 E)。然而,正如 WB 和 qPCR 所示,MTHFD2 敲低并没有改变 PEBP1 的蛋白质或 mRNA 水平(图 5F 和 G)。这些发现表明,MTHFD2 可能通过与 PEB...
Expression of MT-CO1 in the lymph nodes was low in younger mice but high in older mice. In other immune organs (spleen and thymus), MT-CO1 expression was low in older MRL/lpr mice. Lower mRNA expression and higher MDA levels were observed in the brains...
线粒体MtCo1mRNA表达量检测方法如下: 使用TRIzol试剂(Sigma-Aldrich公司,93289)从培养的细胞中提取总RNA。反转录反应使用HiScript IIQ Select RT SuperMix for qPCR(含gDNA清除剂)(Vazyme公司,R233-01)将RNA转录成cDNA。为了评估线粒体编码的mRNA,使用随机六聚体引物进行cDNA的反转录(42℃2分钟;50℃60分钟;85...
COX1抗体, MTCO1抗体, Rabbit Anti-COX1/MTCO1 Polyclonal Antibody 价格￥1500 起批量≥1支 最小起订1支 供货总量999支 发货地址上海市浦东新区 建议售价￥1500/支 更新日期2025年03月28日 产品规格50ul,100ul 即时洽谈立即询价查看联系方式 收藏产品发送留言 ...
Einarsdottir et al,Immunogenicity of Comirnaty Omicron XBB.1.5booster cOVID-19mRNA vaccine in long-term survivorsafter allogeneic hematopoietic stem cell transplantation.Scientific reports 2024 Kellner et al,The Nuclear Speckles Protein SRRM2 /s Exposed on the Surface of Cancer Cells.Cells 2024 ...
cDNA合成反应Artemia mRNA25μl(500ng)DEPC H2O 30μl10μM AP5μl将上述混合物在70℃孵育10分钟,然后在冰上放置1-2分钟。通过在10000rpm离心10秒来收集挥发的液体。将下述物质加入到上述RNA混合物中,以产生cDNA溶液10x PCR缓冲液10μl25mM MgCl210μl10mM dNTP5μl0.1mM DTT10μl然后混合上述得到的cDNA溶...
, and the mRNA levels were determined via a qPCR instrument. The relative expression level of the target gene was normalized to that of β-actin. The primers spanned exons for each gene. The primer sequences are shown in Table S1. Cell counting Kit-8 (CCK-8) A CCK-8 (Beyotime, ...
On the contrary, a significant relationship was seen between the relative expression levels ofTFAMandNRF1and theMT-CO1genes at the stages of metaphase I (MI) and MII (p= 0.03 andp= 0.002). A relationship exists between the transcript expression levels ofTFAMandNRF1, andMT-CO1genes in ...
📖 What we’re reading Darcy Ward, lead author and policy analyst at TBI, sets out that if the UK is to become a world leader in mRNA technology, then it needs to recapture the energy behind the Covid vaccine. She writes that it is now the role of the Department of Science, ...