Inactivation of the ZIP4 gene in Arabidopsis leads to a complete blockage of the ZMM pathway responsible for Class I crossovers and, consequently, a strong reduction of plant fertility due to crossover scarcity29. Earlier studies have shown that a knock-out mutation in the FANCM gene restores ...
Human MSH6, which stands for mutS homolog 6, is a member of the MMR family that is responsible for the repair of post-replicative mismatched DNA bases. It is also one of the proteins with gene mutations that are associated with a high risk of developing Lynch syndrome, leading to a large...
UCH stands for the ubiquitin C-terminal hydrolase domain. F, C terminus of USP10 binds MSH2. Both wild type and catalytically-dead mutant of USP10 bind MSH2. Bacterially purified GST-MSH2 was incubated with 293T cell lysates transfected with Flag-empty vector (F-vector) or indicated USP10...
First, we analyzed all DNA samples by a well-established and quick method, by methylation-specific PCR (MSP) for methyla- tion of mismatch repair gene promoters most frequently involved in human cancers, namely MLH1, MSH6, PMS2, and MSH2. The findings were validated using a highly sensitive...