Moss RNA was isolated using the RNAeasy kit (Qiagen) starting with 100 mg of fresh protonema. We obtained doubled-stranded cDNAs corresponding to mRNAs expressed in 6-day-old protonema cultures using the SMART-PCR cDNA Synthesis Kit (Clontech). The primers PpMSH2#4 and PpMSH2#2 (CACCAAC...
α-MSH (Bachem AG) or ClpB protein (Delphi Genetics) were diluted to 0.5 mg ml−1 in 10 mM sodium acetate buffer, pH 5.0 (GE Healthcare), and were covalently coupled on the sensor chips CM5 (GE Healthcare) by using the amine coupling kit (GE Healthcare). All measures ...
unique copy of each chromosome (AC,n = 19) so that (almost) all crossovers observed in allohaploids involve homoeologues. Here, we show that regular meiosis is achieved inB. napuseven when the number of functionalMSH4copies is reduced to a minimum. By contrast, we demonstrate that th...
Studies on the skin-related activities of marine algae have been conducted in developed countries, such as the Netherlands, Norway, Germany, England, and France. These studies have emphasized the usefulness of marine algae as functional cosmetic materials [24]. Leathesia difformis (L.) Areschoug...
Rico-Méndez MA, López-Ceballos AG, Moreno-Ortiz JM, Ayala-Madrigal MdlL, Gutiérrez-Angulo M, Ramírez-Ramírez R, González-Mercado MG, González-Mercado A. Intronic Variants in the MSH2 (rs2303426 and rs10179950) and PMS2 (rs2286681 and rs62456178) Genes Are Not Associated with Colorecta...