After washing three times with wash buffer, 35 μL of read buffer T with surfactant (Meso Scale Discovery) was added to each well and the plate was imaged on a Sector Imager 6000 (Meso Scale Discovery) according to manufacturers’ instructions. The following antibody combinations were used: 4...
MSD 384-well plates (Meso Scale Discovery) were coated overnight at 4°C with 10 μL of coating antibody in a carbonate-bicarbonate coating buffer (15 mM Na2CO3/35 mM NaHCO3, pH 9.6) per well. Plates were ...
MC4R活性激动剂至少部分通过减轻体重来改善血糖稳态,然而其他黑素皮质激素受体可能有潜力治疗与肥胖相关的葡萄糖稳态异常。 Intracellular cAMP levels were determined by an electrochemiluminescence (ECL) assay (Meso Scale Discovery, MSD). CHO-K1 ...
高通量多蛋白检测仪 SECTOR® Imager 6000,SECTOR®Imager 6000The SECTOR Imager 6000 uses ultra-low noise charge-coupled device (CCD) camera with custom-designed telecentric lenses for rapid detection using all MULTI-ARRAY®or MULTI-SPOT
(Meso Scale Discovery) was added to each well and the plate was imaged on a Sector Imager 6000 (Meso Scale Discovery) according to manufacturers’ instructions. The following antibody combinations were used: 4 μg/mL pAb147 (coating) / 1:1,000 MAB2166 (detection) / 1:1,000 anti-mouse-...
SECTOR Imager 2400,MESO SECTOR S 600MM,SECTOR Imager 6000,MESO QuickPlex SQ 120MM,MESO QuickPlex SQ 120,MESO SECTOR S 600 Plate Type 96-well Plate Surface Standard Usage Statement For Research Use Only. Not for use in diagnostic procedures. ...
MESO QuickPlex SQ 120 , SECTOR Imager 2400 , SECTOR Imager 6000 , MESO SECTOR S 600 Plate Type 96-well Capture Antibody IFN-γ: Rat Monoclonal IL-1β: Mouse Monoclonal IL-2: Rat Monoclonal IL-4: Rat Monoclonal IL-5: Rat Monoclonal IL-6: Rat Monoclonal KC/GRO: Rat Mon...
MESO QuickPlex SQ 120 , SECTOR Imager 2400 , SECTOR Imager 6000 , MESO SECTOR S 600 Plate Type 96-well Capture Antibody IFN-γ: Mouse Monoclonal IL-1β: Mouse Monoclonal IL-4: Mouse Monoclonal IL-5: Rat Monoclonal IL-6: Mouse Monoclonal KC/GRO: Rabbit Polyclonal IL-10:...
the cells were lysed and 2.5 nM TAG ruthenium-labeled cyclic AMP (MSD) was added. After 90 minutes, cyclic AMP levels were determined by ECL detection using Sector Imager 6000 reader (MSD). NDP-MSH was used as the reference compound. Maximal cAMP stimulation level was 12 to 20 folds highe...
产品名称:S-PLEX 96-Well SECTOR Plate 货号:L45KA 品牌:MSD Specifications Instrument(s):SECTOR Imager 2400 , SECTOR Imager 6000 , MESO QuickPlex SQ 120 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , MESO SECTOR S 600MM Plate Type:96-well Plate Surface:Standard Usage Statement:For Researc...