检测指标:For AMPK MSD assays Standard Bind plates (Meso-Scale Diagnostics, L15XA-3) were coated overnight at 4 °C with 120 ng/well of AMPKα antibody (Abcam, 80,039). Each well was washed once with 200 μL 1× MSD Tris Wash Buffer (Meso-Scale Diagnostics, R61TX-1) then 200 μL...
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检测指标:An MSD western-replacement method (Meso Scale Discovery) using specific anti-alpha smooth muscle actin (alpha-SMA) antibody was used to quantify SMA in protein lysates. Samples (25 uL) were added to multi-array 96-well plates (high bind, Meso Scale Discovery) and incubated for 2 h...
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diluted in 1% MSD Blocker A, were added to respective wells and incubated for 1.5 h at 37 °C. Wells were then washed with 1× Tris Wash Buffer, once, then 50 ng of MSD Sulfo-Tag antibody (Meso-Scale Diagnostics, R32AB-1) was added to each well and incubated at 37 °C for 1 ...
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