检测指标:For AMPK MSD assays Standard Bind plates (Meso-Scale Diagnostics, L15XA-3) were coated overnight at 4 °C with 120 ng/well of AMPKα antibody (Abcam, 80,039). Each well was washed once with 200 μL 1× MSD Tris Wash Buffer (Meso-Scale Diagnostics, R61TX-1) then 200 μL...
样品处理:Liver tissue samples were homogenized in cell lysis buffer (Cell Signaling). 检测指标:For AMPK MSD assays Standard Bind plates (Meso-Scale Diagnostics, L15XA-3) were coated overnight at 4 °C with 120 ng/well of AMPKα antibody (Abcam, 80,039). Each well was washed once with ...
规格:96孔 品牌:MSD 特殊需求:L15XA-3 货号:L15XA-3 单位:块 数量:20 供应商报价详情 序号:01 是否成交:成交 供应商名称:上海优宁维生物科技股份有限公司 报价时间:2023-02-13 14:48:00.0 最新中标 中标单位:上海优宁维生物科技股份有限公司 共0条查看 江苏省疾病预防控制中心(江苏省公共卫生...
Si4214DDY-T1-GE3、B32921C3103M289、NCP1400ASN19T1G、CXA3767R、CY7C1021BV33L-15ZI、LC4128C-75TN100I、MSD106CHL-LF-Z1、IDT72V225L15PFI、GF-9300-730I-B2、IS43TR16128C-15HBL、B45197A2107M509、EFM8SB20F32G-B-QFN32R、LH-06034P1-B2-A01、TS5A3167YZPR、MMD-12EZ-R82M-V1、URB2412LD-...
检测指标:For AMPKMSD assays Standard Bind plates (Meso-Scale Diagnostics, L15XA-3)were coated overnight at 4 °C with 120 ng/well of AMPKα antibody (Abcam, 80,039). Each well was washed once with 200 μL 1× MSD Tris Wash Buffer (Meso-Scale Diagnostics, R61TX-1) then 200 μL ...
Meso Scale Discovery(MSD)的电化学发光技术是基于Elisa原理的升级,在96/384微孔板(石墨材质,背面带有电路)的板底包被捕获抗体,加入待测样本,加入三联吡啶钌标记的检测抗体,形成双抗体夹心复合物,将微孔板放入MSD仪器中,微孔板通电后,三联吡啶钌发生化学反应, 由[Ru(bpy) 3 ]3+变成 [Ru(bpy) 3 ]2+,再回到...