读取缓冲液应在去离子水中稀释。稀释读取缓冲液的制备或读取缓冲储备的蒸发的差异可导致板之间绝对信号值的差异,所以现在MSD主推无需稀释的2X MSD GOLD Read Buffer。 2. 移液变异性:由于设备或操作人员之间的差异,分析变异性通常与移液差异有关。确保移液管已校准并且使用了正确的移液管tips。在每一个分液步骤之...
MSD GOLD Read Buffer A MSD GOLD products provide a rapid and convenient method for the development of new immunoassays and the transfer of existing ELISAs utilizing the benefits of MSD’s highly sensitive electrochemiluminescence (ECL) technology. Read buffer is an essential component of MSD ...
MSD Read Buffer T (4x), Surfactant Free在使用SULFO-TAG™ Conjugated Biotinylated IgG (BTI)和MSD GOLD™ 96-well High Bind Avidin涂层板时进行了测试。 MSD Read Buffer T (4;MSD;试剂盒;缓冲液;电化学;公司简介上海优宁维生物科技股份有限公司,自2004年成立以来一直专注于抗体及相关产品,是国内专业...
4. 信号检测 加入Sulfo tag标记的大鼠抗小鼠 IgM 抗体，孵育 1 小时。该抗体可与 K2 抗体特异性结合，由于其带有Sulfo tag标记，在后续检测中可产生电化学发光信号。再次洗涤孔板，去除未结合的标记抗体，然后加入 MSD GOLD Read Buffer A（Meso Scale Discovery 产品）。之后，立即将孔板放置在 MSD Quickplex SQ ...
6.当必要时,可使用2倍浓度的MSD读取缓冲液T(或MSD Gold Read Buffer)来防止分析背景信号过于接近仪器背景信号。如使用1X读缓冲液获得低特异性信号,则可以使用2X读缓冲液来增加信号并减少板间和运行间的可变性。 7. 在特定基质如血清、血浆、尿液或脑脊液CSF中...
英文别名: MSD GOLD Read Buffer A 1000 mL中文名: 中文别名: CBNumber: CB19713418 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 1 MSD GOLD Read Buffer A 1000 mL化学性质 安全信息MSD GOLD Read Buffer A 1000 mL性质、用途与生产工艺 MSD GOLD Read Buffer A 1000 mL ...
controls and samples. After incubation for two hours, the plates were washed three times and detection antibody was added at 2 μg/mL (MSD SULFO-TAG™ Anti-Human IgG Antibody). Plates were incubated for one hour and washed three times. MSD GOLD™ Read Buffer B was added (150 μL) ...
Using the MSD GOLD Read Buffer, the applied voltage causes the captured TURBO-TAG labels to emit light. V-Plex Technology: The “V” stands for validated, since V-Plex plates undergo an accurate validation and are shipped with a certificate of analysis (CofA). The validation includes: Percent...
h. Wells were washed three times with 1×Tris Wash Buffer, 150 μL of 1× MSD Read Buffer (Meso-Scale Diagnostics, R92TC-2) was added to each well and plate read on an MSD plate reader (Meso-Scale Diagnostics, Sector S600).For ACC MSD assays, MSD Streptavidin gold plates (Meso-...
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