We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by...
(429) BseYI (425) BbsI (421) SacII (375) MspA1I (374) EcoO109I * (209) AhdI (180) StyI (146) BstEII (141) BbvCI - Bpu10I (136) BssSI - BssSαI (73) BanII (61) BsiHKAI (50) ApaLI (46) BtsI - BtsαI (23) BclI * (20) Start (0) mScarlet3 mScarlet3 687 bp ...
名称: pCMV-mScarlet3-P4HB(human)-Neo 别称: NM_000918.4;CLCRP1; DSI; ERBA2L; GIT 基因长: 1527bp 规格: 默认发少量干粉质粒(现货) 大提液体质粒(期货) 质粒宿主: 哺乳细胞 质粒用途: 蛋白表达 片段类型: ORF 片段物种: 人 原核抗性: Kan 筛选标记: Neo/G418 荧光标记: 红色 启动子: CMV 复制子...
S1287-9 AAV2/9-IBA1-MasterRNAi155(NC)-mScarlet3-miR-9.T-miR-129-2-3p.T-WPRE-pA 价格: ¥500.00~6000.00 评论:0条 收藏:0 腺相关病毒包装规格: 金标AAV 5ul ≥1E+13V.G./mL 金标AAV 20ul ≥1E+13V.G./mL 金标AAV 50ul ≥1E+13V.G./mL...
S1287-11 AAV2/11-IBA1-MasterRNAi155(NC)-mScarlet3-miR-9.T-miR-129-2-3p.T-WPRE-pA 价格: ¥500.00~6000.00 评论:0条 收藏:0 腺相关病毒包装规格: 金标AAV 5ul ≥1E+13V.G./mL 金标AAV 20ul ≥1E+13V.G./mL 金标AAV 50ul ≥1E+13V.G./mL...
International Journal of Molecular Sciences Article LSSmScarlet2 and LSSmScarlet3, Chemically Stable Genetically Encoded Red Fluorescent Proteins with a Large Stokes' Shift Oksana M. Subach 1, Anna V. Vlaskina 1, Yulia K. Agapova 1, Kiryl D. Piatkevich 2,3,4 , Maxim V. Patrushev 1, ...
Recently, two bright monomeric red fluorescent proteins, mRuby3 and mScarlet-I, have been developed. These proteins offer much improved physical properties compared to previous generations of monomeric red FPs that should help facilitate more general adoption of Green/Red FRET. Here we assess the ...
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The faster maturation and higher pH stability in acidic conditions of LSSmScarlet3 compared to LSSmScarlet result in a 2.2-fold higher brightness of LSSmScarlet3 in lysosomes in fusion with lysosomal associated membrane protein 3 (LAMP3). The monomeric properties of LSSmScarlet2 and LSSmScarlet3 ...