Alexa Fluor 488 and Cy3-conjugated secondary antibody and DPAI (Sigma-Aldrich) were applied for incubation for visualization following treatment with the primary antibody CD31 (1:200, Abcam), as well as alpha-smooth actin (α-SMA) (1:50, Abcam), the angiogenesis markers. Then, a ...
(Sigma-Aldrich, St. Lois, MO, USA), Syntenin (clone EPR8102; Abcam, Cambridge, UK), CD81 (clone 5A6; Biolegend, San Diego, CA, USA), CD9 (VJ1, kindly provided by Francisco Sánchez-Madrid) and the absence of cytochrome C (clone 6H2.B4; Biolegend) to exclude cellular protein ...
The aminoethylanisamide (AA)-PEG-vectorized exosomes loaded with paclitaxel could target the sigma receptor and improve anti-lung cancer outcomes [155]. Exosomes combined with various nanoparticles is a novel targeted system for drug delivery. A33-positive exosomes loaded with doxorubicin could bind to...