Immunofluorescence images of spleen T cells and B cells were obtained using an Olympus VS200 fluorescence microscope and analyzed with OlympusVIA software (Olympus, Japan). Statistical analyses All data were statistically analyzed using GraphPad Prism 8.0.2. The Kolmogorov–Smirnov and Shapiro–Wilk ...
Cartilage-specific gene expression analysis in vitro. Real-time polymerase chain reaction (RT-PCR) analysis was performed using an ABI 7300 RT-PCR system (Applied Biosystems, Foster City, CA, USA) with SYBR Green PCR Master Mix (Toyobo, Osaka, Japan) to determine the gene expression of ...
www.nature.com/scientificreports OPEN In vivo vascularization of MSC- loaded porous hydroxyapatite constructs coated with VEGF- received: 21 July 2015 accepted: 18 December 2015 Published: 22 January 2016 functionalized collagen/heparin multilayers Kai Jin1, Bo Li2, Lixia Lou1,Yufeng Xu1,...
An optical microscope (Olympus IX 70, Tokyo, Japan) was applied to obtain images of the stained sections. Immunofluorescence analysis After the harvested sections were deparaffinized and rehydrated, 1.5% goat serum (Merck-Millipore) was utilized for blocking for immunofluorescence (IF). Alexa Fluor...
Samples were observed using transmission electron microscopy (TEM; H7650, Hitachi, Tokyo, Japan). Oxygen and glucose deprivation (OGD) HUVECs were seeded onto 6-well plate, and OGD was conducted when the confluency reach to 90%. Briefly, the culture medium of the HUVECs was replaced with ...
qPCR was performed using TB Green Premix Ex Taq II (RR820A, Takara, Japan) on a CFX Connect real-time PCR detection system (Bio-Rad, CA, USA). The data were analyzed using Bio-Rad CFX 2.1 in triplicate. 2.10. Statistical analysis All observers were blinded to the experimental group ...
In addition, Crohn's fistula (Alofisel/Takeda) has been multiparametric immune-monitoring tools have approved since 2018 in Europe, Japan and to be set up to characterize the patient immuno- Canada. The first successful autologous logical status and the various immune cell s ubsets MSC(...
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In addition, Crohn's fistula (Alofisel/Takeda) has been multiparametric immune-monitoring tools have approved since 2018 in Europe, Japan and to be set up to characterize the patient immuno- Canada. The first successful autologous logical status and the various immune cell s ubsets MSC(...