Mesenchymal Stem/Stromal Cells (MSC) are a promising cell type for cell-based therapies - from tissue regeneration to treatment of autoimmune diseases - due to their capacity to migrate to damaged tissues, to differentiate in different lineages and to their immunomodulatory and paracrine properties. ...
Microphotographs of sections were taken under ×100/×200 magnification in 3 random fields of view (FOV) per section (DMi8, Leica, Germany). Vessel counts included CD31-positive structures per FOV; αSMA staining was used to detect large vessels and myofibroblasts in granulation tissue. Structure...
The dissected areas were collected with a sterile 1.5-mL polypropylene tube (Eppendorf, Hamburg, Germany). The obtained samples (n≈ 50 per tube) were lysed in appropriate buffer and used for downstream applications: Western blotting, solid-phase dot-ELISA, PicoGreen DNA assay, LDH activity ...