Mesenchymal Stem/Stromal Cells (MSC) are a promising cell type for cell-based therapies - from tissue regeneration to treatment of autoimmune diseases - due to their capacity to migrate to damaged tissues, to differentiate in different lineages and to their immunomodulatory and paracrine properties. ...
Sections were deparaffinized, stained with hematoxylin and eosin (HE), subjected to light microscopy (Leica, Germany) and analyzed using a numerical scale [19]. Image-Pro Plus was used to determine the lesion area in the pathological sections, and the pathological score was analyzed as ...
The dissected areas were collected with a sterile 1.5-mL polypropylene tube (Eppendorf, Hamburg, Germany). The obtained samples (n≈ 50 per tube) were lysed in appropriate buffer and used for downstream applications: Western blotting, solid-phase dot-ELISA, PicoGreen DNA assay, LDH activity ...
Microphotographs of sections were taken under ×100/×200 magnification in 3 random fields of view (FOV) per section (DMi8, Leica, Germany). Vessel counts included CD31-positive structures per FOV; αSMA staining was used to detect large vessels and myofibroblasts in granulation tissue. Structure...