Microcarriers are a commonly used, high surface area 3D culture solution combined with traditional bioreactors for allogeneic therapies; however, bioreactors are operated at a much larger scale than that required for personalized medicine. Therefore, a solution combining mid-size, fully closed, single ...
使用CEL-G® Culture MSC ExoPro SFM间充质干细胞外泌体生产无血清培养基搭配同腾新创自研产品CEL-G® Culture Ad60 固定床生物反应器在3D条件下培养hUC-MSCs（人脐带间充质干细胞），经活细胞荧光染色显示hUC-MSCs能在固定床生物反应器中的膜材上进行贴附生长且分布较为均匀，同时细胞在膜上显示出 3D 球状...
在3D培养模式下(推荐配套使用CEL-G® Culture Ad60固定床生物 反应器),外泌体生产的收获阶段,在细胞状态良好的前提下,可每 2-4天更换一次培养基,换液后可多次收获。 三、CEL-G® Culture MSC Basal SFM Kit间充质干细胞无血清基础培养基套装 CEL-G® Culture MSC Basal SFM间充质干细胞无血清基础培...
[9] Kurogi, H., et al., Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions. Biotechnol J, 2021. 16(6): p. e2000558. [10] Kusuma, G.D., et al., Effect of 2D and 3D Culture Microenvironments on Mesenchym...
By using the VitroGel Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective. 3D cell culture process in 20 min –“Just add cells” VitroGel MSC is ready-to-use. Just mix with your cells. MSC 3D scale-up process in 20 min – Hydrogel-cell bead formation ...
使用CEL-G® Culture MSC ExoPro SFM间充质干细胞外泌体生产无血清培养基搭配同腾新创自研产品CEL-G® Culture Ad60 固定床生物反应器在3D条件下培养hUC-MSCs(人脐带间充质干细胞),经活细胞荧光染色显示hUC-MSCs能在固定床生物反应器中的膜材上进行贴附生长且分布较为均匀,同时细胞在膜上显示出 3D 球状。
VitroGel MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydro...
一、CEL-G® Culture MSC Basal SFM间充质干细胞无血清基础培养基CEL-G® Culture MSC Basal SFM间充质干细胞无血清基础培养基（货号：C04.004.001）是同腾新创专为人类间充质干细胞 （mesenchymal stem cells，简称MSC）,包括脐带间充质、骨髓间充质、脂肪间充质等干细胞设计的基础培养基。本培养基为基础...
一方面,细胞移植的形态从单一细胞悬液的给药方式向2D,3D研究方向发展。其中,我们报告了一种细胞聚集体形态与xeno-free重组支架相结合的被称为CellSaic的细胞治疗平台。CellSaic是按照马赛克状将细胞和花瓣状片装配起来的细胞移植形态,以避免细胞聚集体中心坏死。可避免中心坏死的这一点,是其他的3D培养系统无法比拟的...
VitroGel MSC是一种无异种(无动物来源)水凝胶系统,旨在支持间充质干细胞(MSC)的三维(3D)培养。该水凝胶系统可用于制备用于MSC放大的水凝胶细胞珠。MSC放大不需要微载波。 VitroGel MSC可与优化配方一起使用,完全支持MSC的快速扩张。从液氮中直接解冻或从2D培养容器中传代的细胞可以立即与水凝胶溶液混合,用于3D培养或...