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[29] employed an acetonitrile protein precipitation technique, but the method employed a double 96-well plate in sample preparation which increased the cost of analysis. A gradient method with a flow rate of 0.6 mL/min and analysis time of 2.5 min used in the study consumed 87.5% more of ...
All of these peptides were spotted after every 7 s on the LC−MALDI plate with α−Cyano−4−hydroxycinnamic acid (CHCA). The flow rate of the solvent was 1.6 µL/min. The total run time was 36 min including equilibration. 2.6. MALDI TOF/TOF Analysis A 4800 Plus MALDI TOF/...
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Young leaves and roots, which were cut into smaller fragments to increase their contact surface with the extractor (water), were used for preparing the aqueous extracts by infusion. The infusion was prepared by pouring boiling water on the leaves at a rate of 133.2 g for every two liters ...
Mushrooms have a long history of use as food and medicine. They are rich in various nutrients and bioactive compounds, particularly phenolic compounds. In this study, ten mushroom species were selected, and solvent extraction using 80% ethanol was used to extract phenolic compounds. Total phenolic...