All other analytical-grade chemicals were purchased from HiMedia (Maharashtra, India). 2.2. Instrumentation UPLC–QTOF–MS/MS analysis was conducted using an Agilent 1260 Infinity II LC System coupled to an Agilent 6530 LC/Q-TOF instrument procured from Agilent Technologies (California, USA). ...
This strategy represents a suitable approach for the screening, with high confidence, of several metabolites present in crude extracts and future works will focus on testing the effects of experimental conditions (i.e., media composition, co-cultivation with other microbes, etc.) on the production...
4.3.2. Culture Media The Sabouraud Dextrose Agar (SDA, KASVI, Laboratorios Conda S.A., Madrid, Spain) solid medium, prepared according to the manufacturer’s instructions, and the double concentrated Sabouraud Dextrose Broth (SDB, HIMEDIA, Mumbai, India) liquid medium, were used for the antifun...
4.3.2. Culture Media The Sabouraud Dextrose Agar (SDA, KASVI, Laboratorios Conda S.A., Madrid, Spain) solid medium, prepared according to the manufacturer’s instructions, and the double concentrated Sabouraud Dextrose Broth (SDB, HIMEDIA, Mumbai, India) liquid medium, were used for the antifun...
Fungal isolates were sub-cultured on SDA agar (HiMedia, Mumbai, India) plates at 25 °C for 5–7 days until sufficient fungal growth was observed. Appropriate fungal mycelia were transferred into the Eppendorf tube containing 300 µL deionized water and 900 µL of 100% ethanol (Sigma Aldr...
Strains identified as Aspergillus flavus were inoculated onto Saboraud dextrose agar (SDA) (HiMedia, Mumbai, India), where they were cultured at 25 ◦C for 5–7 days. 3.2.2. MALDI-TOF MS A. flavus Identification Fungal isolates were sub-cultured on SDA agar (HiMedia, Mumbai, India) ...
Twofold serial dilutions of EOs were prepared in Mueller–Hinton (MH) broth (HiMedia). To each well, 5 µL of the inoculum (5 × 105 CFU/mL) was added. Each plate contained growth and sterile control wells. The microtiter plates were incubated at 37 ◦C for 20 h and results were...