MS-based proteomics is expanding its role as a routine tool for biological discovery. Nevertheless, the task of accurately and precisely quantifying thousands of analytes in a single experiment remains challenging. In this study, the diagnostic accuracy of three popular data-dependent methods for ...
原名:SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogenPhytophthora infestans 译名:基于SWATH-MS定量蛋白质组学分析揭示马铃薯晚疫病菌PAMP诱导免...
ProteomicsLiquid chromatography coupled with mass spectrometry (LC-MS) has been widely used for profiling protein expression levels. This chapter is focused on LC-MS data preprocessing, which is a crucial step in the analysis of LC-MS based proteomics. We provide a high-level overview, highlight...
Unlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological changes and disease states. The objective of this study was to develop a robust and comprehensive ...
原名:iTRAQ and PRM -based proteomics analysis for the identification of differentially abundant proteins related to male sterility inms-7mutant tomato (Solanum lycoperscium) plants 译名:基于iTARQ和PRM蛋白质组鉴定ms-7番茄突变...
In common with other omics technologies, mass spectrometry (MS)-based proteomics produces ever-increasing amounts of raw data, making efficient analysis a principal challenge. A plethora of different computational tools can process the MS data to derive
After analysis of the statistically informed MN, clusters were selected based on their node size. Using this criteria, 10 clusters were selected, herein named MN1-MN10. A total of 21 compounds were evidenced in the selected clusters and assigned biomarker potential. Figure6shows the selected clus...
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Proteomics DB(https://www.proteomicsdb.org/)和 Human Protein Atlas(https://www.proteinatlas.org/) 数据库中的蛋白表达信息。前者来源于已公开的质谱检测数据,后者主要来源于RNA测序和抗体相关实验的检测数据。 2、抗体选择 抗体是决定IP实验是否成功的最关键因素。目前各种常见的标签抗体通常都具有良好的特异性...
BERGLUND L, BJöRLING E, OKSVOLD P, et al. 2008. A genecentric Human Protein Atlas for expression profiles based on antibodies. Mol Cell Proteomics [J], 7: 2019-2027. BERTERO A, BROWN S, MADRIGAL P, et al. 2018. The SMAD2/3 interactome reveals that TGFβ controls m(6)A mRNA me...