The bioinformatics analysis found that the CarG element existed in the promoter region of COMT gene of many familiar vertebrates, including of human, rhesus macaque, chimpanzee, etc. The results of RT PCR assay further showed that MRTF-A could enhance the transcription level of COMT. These ...
(westernblot)法检测皮肤组织,皮肤成纤维细胞中COLI,MRTF-A/SRF和平滑肌激动蛋白(smoothmusclealpha-actin,α-SMA)的表达情况,同时检测皮肤成纤维细胞中TGF-b1/Ras同源基因家族成员A(rashomologgenefamily,memberA,RhoA)/RhoA相关蛋白激酶(rho-associatedproteinkinase,ROCK)信号因子的表达情况,双荧光素酶报告基因实验法...
1.4 质粒转染 MRTF-A过表达质粒及空载购于Addgene。肾小球系膜细胞于孵箱培养24 h后开始转染实验。转染步骤按照Lipofectamine® LTX & Plus Reagent说明书进行。转染24 h后,无血清培养基培养12～16 h,然后再改换含AGEs(100 mg·L-1)的培养基培养24 h或1 h。 1.5 siRNA干扰实验 MRTF-A siRNA构建于吉玛基...
表 1 qRT-PCR 引物序列 基因 Gene Table 1 qRT-PCR primer sequence 正向引物(5' -3') Forward Primer(5' -3' ) 反向引物(5' -3') Reverse Primer(5' -3' ) Gm33780 TTGTCTCACTTCCCTTTCTCTGC TCCAATGCTCAAACTCCTGCT Gm3871 TCCAGTTCTCTCAGGCATTGT TGAGGGGAAATGGAACACCG Hic2 ACTGGTTGACCCGTCC...
Following actin polymerisation downstream of Rho signalling, or mechanosensing, MRTF-A is released and functions as a signalling molecule partnering serum response factor (SRF) and influencing gene expression via CARG elements in the promoter region of genes. Genes expressing CARG like elements induced ...
Knockdown of F3 (tissue factor (TF)), a target gene regulated selectively through YAP, blocked cell invasion and migration, whereas knockdown of HBEGF (heparin-binding epidermal growth factor-like growth factor), a gene selectively induced through MRTF-A, prevented cell adhesion in response to ...
Two members of the myocardin protein family, myocardin-related transcription factor (MRTF)-A and MRTF-B are co-activators of serum response factor (SRF). We recently reported that MRTF-A/B activates the transcription of several actin cytoskeletal/focal adhesion genes SRF dependently, thereby enhanci...
Quantitative proteomics and transcriptomics analyses linked the differential MRTF-A activities to the expression of the ubiquitin-like modifier interferon-stimulated gene 15 (ISG15), which is known to modify focal adhesion and cytoskeletal proteins. The malignant breast cancer cell line MDA-MB-231 ...
of histone amino termini not only correlates with chromosome remodeling and functional status, but also provides an important regulatory platform for gene transcription. Among these modifications, histone lysine methylation occurs on histone H3 at Conflict of interest The authors declare that they have ...
mRNA expression was normalized to the expression of the housekeeping gene cyclophilin. Melt curve analysis was performed to verify that a single PCR product was obtained for each sample. Immunofluorescence staining For staining of cytoskeletal associated proteins and NICD1, cells were fixed with an ...