Gene therapy is considered a potential treatment for Duchenne muscular dystrophy (DMD). Researchers have been working on this for many years to find effective therapeutic targets. Here, we found that MRTF-A (myocardin-related transcription factor A) could activate the transcription of L-type Ca2+...
Gene ID (NCBI) 57591 RRID AB_2878822 Conjugate Unconjugated Form Liquid Purification Method Antigen affinity purification UNIPROT ID Q969V6 Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is...
MKL/myocardin-like protein 1 encoded by MKL1 interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific...
RhoARashomologgenefamilymemberARas同源家族基因成员A ROCK Rho-associatedcoiled-coilforming proteinkinase Rho激酶 MRTFmyocardin-relatedtranscriptionfactor心肌素连接转录因子 SRFserumresponsefactor血清应答因子 MRTF-Amyocardin-relatedtranscriptionfactorA心肌素连接转录因子A ...
Following actin polymerisation downstream of Rho signalling, or mechanosensing, MRTF-A is released and functions as a signalling molecule partnering serum response factor (SRF) and influencing gene expression via CARG elements in the promoter region of genes. Genes expressing CARG like elements induced ...
Gene ID (NCBI) 57591 RRID AB_2878822 Conjugate Unconjugated Form Liquid Purification Method Antigen affinity purification UNIPROT ID Q969V6 Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is...
表 1 qRT-PCR 引物序列 基因 Gene Table 1 qRT-PCR primer sequence 正向引物(5' -3') Forward Primer(5' -3' ) 反向引物(5' -3') Reverse Primer(5' -3' ) Gm33780 TTGTCTCACTTCCCTTTCTCTGC TCCAATGCTCAAACTCCTGCT Gm3871 TCCAGTTCTCTCAGGCATTGT TGAGGGGAAATGGAACACCG Hic2 ACTGGTTGACCCGTCC...
Knockdown of F3 (tissue factor (TF)), a target gene regulated selectively through YAP, blocked cell invasion and migration, whereas knockdown of HBEGF (heparin-binding epidermal growth factor-like growth factor), a gene selectively induced through MRTF-A, prevented cell adhesion in response to ...
Since the effect of p38/MK2-mediated phosphorylation on MRTF-dependent gene expression could be cell type specific, we also analysed whether the presence of the intact Ser312/Ser333 phos- phorylation sites on MRTF-A is critical for MRTF-A-induced expression of SMA in NMuMG cell line derived...
1.4 质粒转染 MRTF-A过表达质粒及空载购于Addgene。肾小球系膜细胞于孵箱培养24 h后开始转染实验。转染步骤按照Lipofectamine® LTX & Plus Reagent说明书进行。转染24 h后,无血清培养基培养12～16 h,然后再改换含AGEs(100 mg·L-1)的培养基培养24 h或1 h。 1.5 siRNA干扰实验 MRTF-A siRNA构建于吉玛基...