Conversion of Ribosome-Protected mRNA to DNA and Deep Sequencing for Ribosome Profiling in Haloferax volcaniiArchaeaFootprintHigh throughput sequencingRibosomeTranslation efficiencyThe translation of messenger RNA (mRNA) into protein is an essential process for all forms of life. The ability to monitor ...
最近mRNAs不仅用于制备iPSC,还用于使用CRISPR/Cas9技术对所得iPSC进行基因编辑。与质粒DNA相反编码Cas9的mRNA的瞬时递送为进一步编辑cmRNA重编程的iPSCs提供了一个无整合的平台,以引入用于疾病建模的特定基因突变或通过同源定向修复纠正基因突变。这两种方法在再生医学中高度相关。 如前所述,其他无整合重编程方法如使用非...
2020年新冠疫情在全球范围爆发,整个世界对新冠疫苗的需求量日益增大,Moderna及Pfizer/BioNTech的mRNA新冠疫苗陆续上市后,行业逐渐认识到mRNA(信使核糖核酸)新冠疫苗/药物的可行性与优势。 脱氧核糖核酸(DNA)和核糖核酸(RNA)的主要作用是携带和传递遗传信息。这两类分子能够用于疾病的预防、诊断和治疗的,就是核酸药物。核...
mRNA-based therapeutics are widely used for treating various diseases and represent an emerging preventive and therapeutic approach that can be an effective alternative to DNA- and recombinant protein-based therapeutics. Accompanied by the continuous development of in vitro synthesis, purification, modificat...
mRNA-based therapeutics are widely used for treating various diseases and represent an emerging preventive and therapeutic approach that can be an effective alternative to DNA- and recombinant protein-based therapeutics. Accompanied by the continuous development of in vitro synthesis, purification, modificat...
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4. Can DNA act as an information storage medium? 4.DNA可以用作信息存储介质吗? Engineering & Material Science 工程与材料科学 1. What is the ultimate statistical invariances of turbulence? 1.湍流的最终统计不变性是什么? 2. ...
As the subject of basic and applied research for more than 5 decades, mRNA has only recently come into the focus as a potentially powerful drug class able to deliver genetic information. Synthetic mRNA can be engineered to resemble mature and processed mRNA molecules as they occur naturally in ...
the artificially targeted conversion of U-to-Ψ in translation termination codons (UAA, UGA, and UAG) turns them into missense codons87. In particular, the U-to-Ψ change at premature termination codons (PTCs) can inhibit the rapid mRNA degradation triggered by nonsense-mediated mRNA decay (NMD...
The modus operandi for single cell sequencing is the addition of a unique tag (barcode) to the DNA/RNA from each single cell, which in turn allows for highly multiplexed sequencing on a short-read sequencer, like popular machines from Illumina. After sequencing, demultiplexing allows for separati...