在转录起始位点(the start site of transcription)上游,有一段保守启动子序列(consensus promoter sequence)。RNA polymerase 识别并且结合到特异性的启动子上(recognizes and binds specific promoter),打开(melt)转录起始位点附近的DNA双链,启动RNA的从头合成(initiate de novo synthesis of RNA)。RNA polymerase 利用NT...
在mRNA递送领域,多种含胺基的聚酯已被开发用于mRNA递送,包括聚(β-氨基酯)[poly(β-aminoester),PBAEs,4]、氨基聚酯(amino polyester,APEs)和电荷改变释放转运体(charge-altering releasable transporters,CARTs,5和6)等。其中,PBAEs 是经...
一个是NCBI Reference Sequences (RefSeq)区域；一个是每个转录本的Nucleotide界面。
其中DNA是双螺旋结构，两个核糖核酸链相互盘绕，而RNA则是单螺旋结构，只有一条核糖核酸链mRNA以三个碱...
今天给各位翻译的是2021年《 Adv Mater》期刊文章:Leveraging mRNA Sequences and Nanoparticles to Deliver SARS-CoV-2 Antigens In Vivo。摘要新型冠状病毒已经成为世界上的疫情;因此急需一种有效的疫苗。最近…
RNA的二级结构对其功能至关重要。AI可以通过深度学习模型,如循环神经网络(RNN)或转录-翻译模型,预测RNA的二级结构。 代码语言:python 代码运行次数:0 复制 Cloud Studio代码运行 # 示例代码,使用RNAfold进行RNA二级结构预测importsubprocessdefpredict_rna_structure(sequence):command=f"RNAfold --noPS <(echo{sequence...
第一种在mRNA疫苗的设计中最为常用,通常直接选取受用群体内高表达基因的5’UTR,例如人alpha珠蛋白的 5’UTR、鼠源alpha珠蛋白的 5’UTR或猫源DNAH2基因的5’UTR等。 第二种是通过指数富集(SELEX)的方式来实现 5’UTR的系统演化,这种方法耗时且筛选...
Serial analysis of gene-expression (SAGE, Velculescu et al., 1995) is a more rapid method to obtain partial sequence information from a very large set of expressed genes, e.g. differences in gene expression profiles in normal and cancer cells are identified by hundreds of differentially expres...
et al. A facile method for the removal of dsRNA contaminant from in vitro-transcribed mRNA. Mol. Ther. Nucleic Acids 15, 26–35 (2019). Article PubMed PubMed Central Google Scholar Thess, A. et al. Sequence-engineered mRNA without chemical nucleoside modifications enables an effective ...
The DNA sequence is: 5'-ATTCGACC-3'. What will be the sequence of the complementary strand using this strand as the template? The sequence of one strand of a DNA molecule is 5'-AGCGCT. What is the sequence of the other...