mRNA提取试剂盒(mRNA Isolation Kit)主要包括植物、动物、微生物基因组DNA的提取与鉴定,哺乳动物组织总RNA的提取与鉴定,质粒DNA的提取与鉴定,PCR扩增,分子杂交技术,限制性内切酶消化,分子克隆全过程和基因文库的构建等实验项目的原理及步骤。 mRNA提取试剂盒(mRNA Isolation Kit)产品及特点 ...
microRNA Isolation Kit——分离miRNA或靶mRNA microRNA(miRNA)虽然只有22个左右的碱基,但是在基因表达调控中起着至关重要的作用,参与细胞分化、个体发育,且与 多种疾病 相关。因此对于miRNA的研究越来越多。传统的
Dynabeads® mRNA DIRECT™ Micro Kit Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA preps Dynabeads® Oligo (dT)25-61002 Dynabeads® Oligo (dT)25-61005 FastTrack® MAG mRNA Isolation Kits PureLink miRNA Isolation Kit PureLink...
Dynabeads® mRNA DIRECT™ Kit Dynabeads® mRNA DIRECT™ Micro Kit Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA preps Dynabeads® Oligo (dT)25-61002 Dynabeads® Oligo (dT)25-61005 FastTrack® MAG mRNA Isolation Kits PureLin...
Little is known about the relationship between miRNA and mRNA expression in Alzheimer’s disease (AD) at early- or late-symptomatic stages. Sequence-based target prediction algorithms and anti-correlation profiles have been applied to predict miRNA targe
After extraction of both miRNA and mRNA, the Qiagen RNeasy MinElute Cleanup Kit (Qiagen, Valencia, CA, USA) was used to remove any contaminating DNA and concentrate samples. RNA concentration was measured using the NanoDrop 2000c spectrophotometer (Thermofisher Scientific, Waltham, MA, USA). ...
miRNAs sequencing and assembly We constructed six cDNA libraries of small RNAs from F1, F2, F3, M1, M2, and M3. After filtering the Rfam database, transcript sequence, and Repbase database, the reads are compared with miRBase to identify and annotate the known miRNA (Table3). The number...
A large proportion of skeletal muscle insulin resistance in type 2 diabetes (T2D) is caused by environmental factors. By applying multiomics mRNA, microRNA (miRNA), and DNA methylation platforms in biopsies from 20 monozygotic twin pairs discordant for T
Top: enrichment of the HNRNPC-bound 3′ UTRs as a function of APAR between sgHNRNPC/sgPABPC4 and sgControl/sgPABPC4 cells; statistics as in Fig. 2a. e, Venn diagram showing the overlap between HNRNPC 3′ UTR targets and AGO2 bound mRNAs (top) or miRNA target mRNAs (bottom), as ...
Conclusion We performed a comprehensive screen for UVB-regulated microRNAs in human diploid fibroblasts, and identified a network of miRNA-mRNA interactions mediating UVB-induced senescence. In addition, miR-101 and Ezh2 were identified as key players in UVB-induced senescence of HDF.Back...