In addition, by predicting retention time for each peptide candidate, it allows selection of several compatible transition candidates that can be monitored simultaneously, permitting MRM. In this chapter, we explain how to go about designing transitions using the web-based transition design tool, MR...
Transition score coefficientDescriptionRelative weighting MS/MS suitability (q) Assesses MS/MS spectrum for suitability in MRM by assessing m/z values of y-ions 50 Peptide coverage (c) Favors fragment ions that represent a greater proportion of the precursor peptide sequence 8 Mass range (r) Cons...
However, using APGC and a more selective transition (421 > 151: 421 corresponds to the [M+H]+ ion of oxychlordane in APGC), allowed the determination of oxychlordane without interference from heptachlor epoxide B. Sensitivity The Xevo TQ-S is a highly sensitive and robust tandem ...
PChopper has been developed to predict peptides that can be produced via enzymatic protein digest; this includes single enzyme digests, and combinations of enzymes. It also allows digests to be simulated in 'batch' mode and can combine information from t
We report XCMS-MRM and METLIN-MRM (http://xcmsonline-mrm.scripps.edu/andhttp://metlin.scripps.edu/), a cloud-based data-analysis platform and a public multiple-reaction monitoring (MRM) transition repository for small-molecule quantitative tandem mass spectrometry. This platform provides MRM tran...
MRM质谱技术应用于靶向定量 张磊 zhanglei123@fudan.edu.cn 只采集目标肽段 多反应监测是对复杂混合物中目标蛋白，目标代谢物进行准确定量的技术手段。作为一种质谱检测的分析方法，具有特异性强、灵敏度高、准确度高、重现性好、线性动态范围宽的突出优点，是目前最灵敏的质谱检测模式，被誉为质谱的“金标准”。选 ...
Under the Data menu, the "Linear Regression" page contains the for linearity result calculating the for each transition (Fig. 6). The user is concentration. Once the user chooses given an option to choose a a scale, the result is shown innosrtmanatll,yloing2t,aobrlelofgo1r0msc...
MRM TRANSITION RATIO 下载积分: 700 内容提示: Are liquid chromatography/electrospray tandemquadrupole fragmentation ratios unequivocalcon rmation criteria?Anton Kaufmann1*, Patrick Butcher1, Kathryn Maden1, Mirjam Widmer1, Kevin Giles2and Diana Urı ´a21Of cial Food Control Authority of the Canton...
The MRM transition pairs were selected by firstly consideration of criteria mentioned in the Kuzyk's work (30) including the amino acid sequences of the peptides must be unique to the target proteins and contain no missed cleavage sites. The targeted peptide should not contain amino acids Cys...
Specific peptides derived from subsequences of the estrogen receptor (ER), the progesterone receptor (PR), and the antigen Ki67 (Ki67) proteins are provided. The peptide sequences and fragmentation/transition ions for each peptide are particularly useful in a mass spectrometry-based Selected Reaction...