Percoll gradient method was used for the isolation of neutrophils from peripheral blood of mice. Briefly, blood samples were collected in tubes with 3.8% sodium citrate, purified by centrifugation and then resuspended in 2 ml of PBS. The cell pellets were then carefully added into a three-lay...
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Purified genomic DNA from the different sequenced strains used for the design of the microarray was extracted (DNeasy; Qiagen), labelled with Cy5 dCTP using the Klenow fragment of DNA polymerase I (BioPrime; Invitrogen, Carlsbad, CA, USA) and used for the normalisation process [43]. Cy5-...