BACKGROUND: Testing for autoantibodies to myeloperoxidase (MPO) and proteinase 3 (PR3) is part of anti-neutrophil cytoplasmic antibodies (ANCA) test that aids the diagnosis of a number of autoimmune diseases including small-vessel vasculitis. We characterized the differences between two automated ...
ObjectiveConcurrent testing for serum antineutrophil cytoplasmic antibodies (ANCA) by indirect immunofluorescence (IF) and by anti-proteinase-3 (PR3)/anti-myeloperoxidase (MPO) antibody assays may identify patients with PR3-ANCA or MPO-ANCA despite a negative IF (IF-negative MPO/PR3-positive); ...
Monocyte subsets respond differently to antibodies directed against MPO and PR3, with anti-MPO but not anti-PR3 leading to increased IL-1β, IL-6 and IL-8 production. In concordance with the observed higher surface expression of MPO on intermediate monocytes, this subset produces the highest ...
The cohort was built using the databases from the Centre Hospitalo-Universitaire (CHU) of Angers and of the CHU of Dijon. The screening period went from May 2004 to May 2012. Each patient presented with a dual MPO-ANCA and PR3-ANCA profile, with at least one of the solid-phase analysis...
抗中性粒细胞胞浆抗体（anti-neutrophil cytoplasmic antibodies，ANCA）是以中性粒细胞和单核细胞胞浆成分为靶抗原的自身抗体，最常见的靶抗原是髓过氧化物酶（myeloperoxidase，MPO）和蛋白酶3（proteinase 3，PR 3）。ANCA 作为小血管炎的生物学标志物，主要存在于ANCA 相关血管炎（ANCA-associated vasculitis，AAV）...
The granulocyte is full of granules each with many different proteins. It was early shown that antibodies from systemic vasculitis patients bind to the alpha fraction containing the azurophil granules. The most important proteins were proteinase 3 (PR3) and myeloperoxidase (MPO). Thus antibodies to...
The main antigen for the c-ANCA is the proteinase 3 (PR3), which is a serine proteinase of the present in primary granules. Antibodies of p-ANCA positive sera are mainly directed to myeloperoxidase (MPO). Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin-G and also ...
1.4.2 MPO-ANCA检测 在上述筛选获得的p-ANCA阳性患者中,使用免疫斑点法检测MPO-ANCA和PR3-ANCA,剔除PR3-ANCA(+)阳性患者,初步筛选MPO-ANCA(+)患者,再经ELISA法定量检测MPO-ANCA:96孔酶标板中每孔包被人MPO抗原2μg,按1∶100稀释患者或正常健康者血清作为一抗,AKP标记的羊抗人IgG经1∶5 000稀释为二抗,其余...
中央气道疾病以及肺部结节样病变在PR3-ANCA阳性患者中更为常见(P<0.05)。寻常型间质性肺病以及支气管扩张在MPO-ANCA阳性患者中更为常见(P<0.05)。肺泡出血、胸腔积液、淋巴结肿大以及肺静脉充血在MPO-ANCA阳性患者中更多见。 结论:在ANCA相关性血管炎患者中肺部受累非常常见,约80%患者肺部CT存在异常。中央气道疾病...
Tests (WG-HAT), in the framework of the International Federation of Clinical Chemistry and Laboratory Medicine (http://www.ifcc.org/ifcc-scientific-division/sd-working-groups/harmonisation-of-autoantibody-tests-wg-hat/), set out to develop standards for anti-MPO and anti-PR3 antibodies [19–21...