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(D) LCI assays showing that MaMKK1 phosphorylates MaMPK6-3 mainly at T216 and Y218 sites. Value is presented as mean ± SE (n = 6). Different letters indicate significant differences as determined by one-way ANOVA at the p <0.05 level. (E) LCI assays showing that MaMKK1 phosphorylate...
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Mutation of Tyr144 in β5 and Asp218/Glu222 in the phosphorylation lip of MPK6 and the equivalent position amino acids in MPK3 and MPK4 produced increased intrinsic kinase activity of these MAPKs in vitro and in yeast. Expression of the MPK4 mutants in transgenic Arabidopsis plants revealed...