CELLCYCLE 一、MPF的发现及其作用 •1970sRao和Johnson发现与M期细胞(Hela)融合的间期细胞染色体发生凝缩，称为早熟凝集染色体(prematurelycondensedchromosome，PCC)。–G1期PCC为单线状，因DNA未复制。–S期PCC为粉末状，因DNA由多个部位开始复制。–G2期PCC为双线染色体，说明DNA复制已完成。•甚至不同类的M期细胞...
CELLCYCLE 一、一、MPFMPF的发现及其作用的发现及其作用 ••1970sRao1970sRao和和JohnsonJohnson发现与发现与MM期细胞期细胞(Hela)(Hela)融合的间期细胞融合的间期细胞 染色体发生凝缩,称为早熟凝集染色体染色体发生凝缩,称为早熟凝集染色体(prematurely(prematurely ...
因而Nurse（1990）年提出了“M期启动调节的普遍机制”这一概念。TheNobelPrizeinPhysiologyorMedicine2001 Prizecitation:"fortheirdiscoveriesofkeyregulatorsofthecellcycle"LelandHartwell,born1939.FredHutchinsonCancerResearchCenter,Seattle,WA,USA.
Control of the higher eukaryote cell cycle by p34cdc2 homologues. The control of eukaryotic cell proliferation can be conceptually divided into two areas; firstly, those processes, normally involving growth factors in hig... CJ Norbury,P Nurse - 《Biochimica Et Biophysica Acta》 被引量: 165发表...
2ChongqingKeyLaboratoryofForage&Herbivorce.SouthwestUniversity,Chongqing400716,China;3BishanCountyAnimalHealthAuthority,Chongqing402760,ChinaCorrespondingauthor.,Mizy163tomAbstractM—phasepromotingfactor(MPF)isanimportantproteaseinregulatingcellcycleprogression,exploretheexpressionofMPFindifferentstagesofspermatogenesisinmice...
In starfish, the activity of a major Ca2+-and cyclic nuleotide-independent protein kinase has been shown to fluctuate in phase with that of MPF along meiotic and mitotic cell cycle (23, 25). Microinjection of 伪-naphthylphosphate (伪-NP), a potent phosphatase inhibitor, increased ...
We conclude that although growing oocytes in pigs have sufficient key cell cycle components for the G 2 to M transition, they remain incapable of converting these components to active maturation-promoting factor (MPF) until growth is virtually completed. Inhibition of phosphatase 1 or 2A induces ...
Germ Cells in Mice A Role for Cyclin A1 in the Activation of MPF and G2–M Transition during Meiosis of Male Germ Cells in MiceA Role for Cyclin A1 in the Activation of MPF and G2–M Transition during Meiosis of Male Germ Cells in Micecell cycle...
并在10h达到G2末期.OAG在浓度为50μmol/L,100μmol/L,150μmol/L时都可激活 PKC,而CalphostinC在300nmol/L时最大限度地抑制PKC活性.50μmol/L的OAG至少1150see没有改变细胞内钙.50μmol/L的OAG在G2期使MPF活性降低,而300nmol/L calphostin C可以使MPF活性升高.在G2早期加入 OAG50μmol/L可以引起...
遍机制”这一概念。TheNobelPrizeinPhysiologyorMedicine2001Prizecitation:for theirdiscoveriesofkeyregulatorsofthecellcycleLelandHartwellTimHuntPaulNurse born1939.born1943.born1949.FredHutchinsonImperialCancerImperialCancer CancerResearchResearchFundResearchFundCenterSeattleClareHallLincolnsInn ...