目的 构建非病毒性基因治疗递送载体(mPEG5k-PCL1.2k)1.4-g-PEI10k,探讨其细胞毒性及对质粒基因(pDNA)的递送性能.方法 通过开环聚合反应制备共聚物mPEG5k-PCL1.2k-OH,将其羟基末端化学转化为N-羟基琥珀酰亚胺(-NHS)生成mPEG5k-PCL1.2k-NHS,随后同枝化PEI10k进行反应生成三元共聚物(mPEG5K-PCL1.2k)1.4-g-...
(mPEG5k-PCL1.2k)1.4-g-PEI10k,a lower cytotoxicity was found at concentration less than 62.5渭g/ml when compared with PEI10k.The transfection efficacy was found to be influenced by both the time needed for transfection,and it was found that 48 hours was better than 24 hours,and as for ...