ELISpot assays employ the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) technique. A polyclonal antibody specific for mouse TNF-α has been pre-coated onto a polyvinylidene difluoride (PVDF)-backed microplate. Appropriately stimulated cells are pipetted into the wells and the microplate...
IX.TROUBLESHOOTING...10 Tel:800-988-1270Email:info@bio-techne2 WebSite:.novusbio/china I.BACKGROUND Tumornecrosisfactoralpha(TNF-α),alsoknownascachectinandTNFSF1A,isthe prototypicligandoftheTNFsuperfamily(1).Itisapleiotropicmoleculethatplaysa centralroleininflammation,immunesystemdevelopment,apoptosis,an...
BAFF/BLyS/TNFSF13B FGF acidic LIF C1q R1/CD93 FGF-21 Lipocalin-2/NGAL, CCL2/JE/MCP-1 Flt-3 Ligand LIX CCL3/CCL4 MIP-1 alpha/beta Gas6 M-CSF CCL5/RANTES G-CSF MMP-2 CCL6/C10 GDF-15 MMP-3 CCL11/Eotaxin GM-CSF MMP-9 CCL12/MCP-5 HGF Myeloperoxidase CCL17/TARC ICAM-1/CD...