Characterization and validation of cortical cell types To assign biological labels to each of these cell types, we first identified cluster-specific marker genes, similar to other single-cell transcriptomic studies11,12 (Fig. 1b, c, Fig. 2, Supplementary Figure 4). Each cell type exhibited simi...
During mouse embryonic development, pluripotent cells rapidly divide and diversify, yet the regulatory programs that define the cell repertoire for each organ remain ill-defined. To delineate comprehensive chromatin landscapes during early organogenesis, we mapped chromatin accessibility in 19,453 single nuc...
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Continuous hybrid cell lines have been generated by the fusion of allogeneically primed marine T lymphocytes with mouse L-cell-derived fibroblasts of line 613. The resulting hybrid clones express one or more T-cell-specific surface components identifiable by radioiodination of intact cells followed by...
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正常肺(D0)和纤维化肺(D21)中分析的间充质细胞数量分别为1,943和3,386个,基于已知的marker基因的表达可将细胞类型分为6种和7种,以t-SNE图和Heatmap展示如Figure2。 image Figure2 单细胞测序揭示MCs聚类信息 ** 然后文章以相同的分析模式分别剖析了各细胞亚型,包括肌成纤维细胞(Myofibroblasts, Figure3)、Col...
Spatially charting molecular cell types at single-cell resolution across the 3D volume is critical for illustrating the molecular basis of brain anatomy and functions. Single-cell RNA sequencing has profiled molecular cell types in the mouse brain1,2, but cannot capture their spatial organization. ...
Single-cell RNA-seq of thymic C57BL/6 iNKT cells We performed droplet-based single cell RNA-seq (scRNA-seq) analysis of PBS57-CD1d-tetramer+cells sorted from the thymi of 8-week old female C57BL/6 mice using the Chromium system (10x Genomics). To enrich for early precursors and develop...
Single-cell RNA sequencing data can unveil the molecular diversity of cell types. Cell type atlases of the mouse spinal cord have been published in recent years but have not been integrated together. Here, we generate an atlas of spinal cell types based on single-cell transcriptomic data, unify...