During neurogenesis, mitotic progenitor cells lining the ventricles of the embryonic mouse brain undergo their final rounds of cell division, giving rise to a wide spectrum of postmitotic neurons and glia1,2. The link between developmental lineage and ce
A single-cell atlas of pig gastrulation as a resource for comparative embryology Article Open access 18 June 2024 Data availability Raw sequencing data are available on ArrayExpress with the following accessions: Atlas: E-MTAB-6967; Smart-seq2 endothelial cells: E-MTAB-6970; Tal1−/− chi...
A single-cell molecular map of mouse gastrulation and early organogenesis Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. 另一篇的姐妹篇,本文测了E6.5到E8.5的老鼠胚胎发育...
(Fig.1A, Additional file2: Fig. S2 and Methods). Input toFLAMESare fastq files generated from the long-read platform. Using the cell barcode annotation obtained from short-read data as the reference, it identifies and trims cell barcodes/UMI sequences from the long reads. After barcode ...
Single-cell RNA-seq coupled to a new functional annotation approach identifies distinct functional states of Th17 cells and the underlying molecular mechanisms that regulate their pathogenicity. Jellert T. Gaublomme,N Yosef,Y Lee,... - 《Cell》 被引量: 143发表: 2015年 加载更多来源...
在这里,我们展示了NuMorph(基于核的形态测量),一组分析工具,用于量化出生后第4天(P4)小鼠大脑注释区域内的所有细胞核和核标记物,在光片显微镜上清除和成像。我们描述了磁共振成像(MRI),以在组织清除脱水步骤引起的收缩之前测量脑体积,使用iDISCO+方法清除组织,包括免疫标记,然后使用市售平台进行光片显...
Annotation of SNPs and short indels reveals variation in gene features Nucleotide variant datasets include SNPs and short indels for each strain of the Mishima Battery, named pgn2v1 (PGN2), bfmv1 (BFM), blg2v1 (BLG2), njlv1 (NJL), chdv1 (CHD), kjrv1 (KJR), swnv1 (SWN), hmi...
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both poly
Single-cell transcriptomics of the E14.5 and P0 cortex We transcriptionally profiled a total of 18,545 mouse neocortical cells at two key times of corticogenesis using Drop-seq: 10,931 cells at embryonic day 14.5 (E14.5) from six biological replicates, and 7614 cells at birth (P0) from th...
Molecular programs that underlie precursor progression in multiple myeloma are incompletely understood. Here, we report a disease spectrum-spanning, single-cell analysis of the Vκ*MYC myeloma mouse model. Using samples obtained from mice with serologica