Mouse RNA-Seq data with two conditions, four samplesPanagiotis Moulos
Gene functionality is closely connected to its expression specificity across tissues and cell types. RNA-Seq is a powerful quantitative tool to explore genome wide expression. The aim of this study is to provide a comprehensive RNA-Seq dataset across the same 13 tissues for mouse and rat, two ...
RNA-seq for the Kallisto processing container.dElapsed time per million (MM) spots/nucleotides for completing the processing of paired read FASTQ files with the Dockerized Kallisto processing container; rs incanddare ther2correlation coefficient linear fit.eDistribution of the number of detected genes...
Raw Counts重分析 suppressMessages(library(pheatmap))suppressMessages(library(ggplot2))suppressMessages(library(EDASeq))suppressMessages(library(edgeR))suppressMessages(library(sva))rm(list=ls())inputDir="./input"outputDir="./output"#1. load data,数据载入datasets=as.data.frame(scan(paste(inputDir,"...
RNA-sequencing was performed on liver samples 6 weeks post surgery. Differential gene expression was assessed using DESeq2 and changes in gene sets using Gene set enrichment analyses (GSEA). Quantitative Real Time PCR was used to validate select differently expressed genes, as well as to assess ...
RNA-Seq Analysis for Mouse GeneticistsP. TvrdikPlant & Animal Genome
Although RNA-seq data, particularly under the auspices of the ENCODE project, have covered a large number of biological specimens, studies on the SG have been lacking. To better appreciate the wide spectrum of gene expression profiles, we isolated RNA from mouse submandibular salivary glands at ...
RNA-seq data yield was a minimum of ~30 million raw reads. Differential gene expression was evaluated using DESeq2 v1.28.1 with sex and batch as covariates, and q < 0.1 as the threshold for DEGs. GSEA85 was carried out in R v4.0.1 as described above. Number of animals per ...
(Fig.3d) after batch correction. Similar to the single-cell data, ST-HSC lied in between LT-HSC and MPP, corresponding to their biological status. Taken together, these results showed that the cells were correctly sorted and both the bulk and single-cell short-read RNA-seq data were of ...
specific trait. However, as the genomic sequence of many of these strains is not available yet, utilization of these resources has been hampered. We demonstrated here that the high throughput transcriptome analysis by RNA-Seq provides an effective way for utilizing the potential of selected strains...