C Body weight of the vehicle (veh)- or rh Bri2 BRICHOS R221E-treated AppNL-G-F (App) and WT mice during the treatment period, presented as mean ± SEM. Two-way ANOVA with Tukey’s correction for multiple comparisons was used to calculate p-values for differences in body weight....
Densitometry was performed using the Multi Gauge v3.2 software (Fuji film Corp), see E17.5 embryos Supplementary Table 1. and newborn pups was The level of set to 100%. αIn 2-tphreoteexipnerreimlateinvtestwo hGeArePmDHoreinthlyasnatoensemWadTe from lysate WT was (WteSsuTtpeldpy,...
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To quantify the linkage signal in terms of more common LOD scores, multipoint parametric linkage analysis of quantitative traits was performed by MQScore_SNP software package[29]. A priori, a diallelic autosomal QTL is supposed to be placed near each marker. Parameters of the model are estimate...
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The integrins are also able to associate with other membrane receptors in multi-molecular complexes that participate in cell activation. In the egg, integrins are the binding partners of sperm surface proteins [1]; they associate with other tetraspanins, mediate connections between them [2,3] and...
2.6. Statistical Analyses All data in this article are reported as means ± SEM. Statistical analysis was performed using SPSS statistics (IBM, Armonk, NY, USA). Results were analyzed using the Kruskal-Wallis test for multi-group comparisons, followed by the Mann-Whitney rank sum test to ...
2.9. Cell Culture, Small Interfering RNA Transfection, and Protein Analysis INS-1 832/13 cells were cultured in RPMI-1640 containing 11.1 mM D-glucose supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Gibco), 100 µg/mL streptomycin (Gibco), 10 mM N-2 hydroxyethylpiperazine-...
Subcutaneous analgesic (Buprenorphine, 1 µL/g, SC, Reckitt Benckiser Pharmaceuticals Inc., Richmond, VA, USA) was injected 20 min before surgery and thereafter for 3 days in addition to topical antibiotic (Multi antibiotic with pain relief, Walgreen, Deerfield, IL, USA). Laser Doppler (Pe...
International Journal of Molecular Sciences Article Investigating Immune Responses to the scAAV9-HEXM Gene Therapy Treatment in Tay–Sachs Disease and Sandhoff Disease Mouse Models Shalini Kot 1,† , Subha Karumuthil-Melethil 2,†, Evan Woodley 1, Violeta Zaric 2,3, Patrick Thompson 4, ...