In this protocol, we describe how to assess the extent and features of infiltrating macrophages by double immunofluorescence. Total macrophage population was determined using an F4/80 antibody, whereas the specific M2-like population was assessed using a CD206 antibody. For complete details on the ...
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 APC-conjugated Monoclonal Antibody (Catalog # FAB25351A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins...
Adding PSTPIP2 expression alleviates liver fibrosis and hepatic inflammation in mice by regulating macrophage polarization.doi:10.1038/s41388-018-0383-0Yang YangWu Xiao-qinLi Wan-xiaHuang Hui-minLi Hai-diPan Xue-yinLi Xiao-fengHuang Cheng
The depletion protocol of CD206+ macrophages (MΦ) in the CD206 DTR mouse-derived endometriosis model. After one week of inoculation of endometrial fragments derived from donor mice (①), the formation of an endometriotic-like lesion in the peritoneal cavity was confirmed in some mice. (②...
(Invtirogen, CA, USA) and according to the manufacturer’s protocol. The cell count, viability and differentiation were noted on triple experimental procedures. IL17 cell pollution was gated in R1 against total CD4 cell populations. Total CD4 cells was gated against the stimulated anti-CD3/CD...
Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms TGF- beta 1, TGF beta1, Tgfb, Tgfb 1, Tgfb1, TGF-beta 1, TGFbeta1, TGF-beta1, TGF-beta-1, TGFβ1, TGF-β1Publication...
Mesenchymal stromal cell-derived extracellular vesicles modulate microglia/macrophage polarization and protect the brain against hypoxia-ischemic injury in neonatal mice by targeting delivery of miR-21a-5p. Acta Biomater. 2020;113:597–613. Article CAS PubMed Google Scholar Zheng J, Zhu L, In II,...
Although our anti-mouse RELM alpha antibody might be of interest to all researchers studying macrophage biology, it will be particularly valuable to the scientists who aim to better understand the process of macrophage polarization. In addition, this new antibody will be useful for the researchers ...
Ninety-six hours post transfection, RNA isolation was performed with TRIzol™ according to the manufacturer’s protocol. cDNA synthesis were carried out using SuperScript™ IV VILO™ Master Mix with the ezDNase kit. To determine the effect on the expression of candidate target genes, primers...
34or cerebral ischemia35. LCN2 can play a role in macrophage polarization and has been shown to deactivate alveolar macrophages in pneumococcal pneumonia where it might facilitate resolution of the inflammatory response36. Thus, LCN2 might provide a means to prevent overwhelming inflammation and ...