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000 protein-coding genes considered to be one-to-one orthologs with high confidence2. However, structural orthology does not equal functional similarity since expression patterns of orthologous genes can deviate substantially across organs and development3. In leukocytes, expression of most orthologous gen...
containing 10% FBS or mAdSC condition medium (CM) containing 10% FBS stimulated with 0.25 μL/well of soluble anti-CD3/CD28 antibodies (Life Technologies AS, Oslo, Norway) and 0.2 μg/mL of recombinant murine IL-2 (PeproTech, Rocky Hill, NJ, USA) for 96 h at 37 °C....
ABN-H00115669-K H00115669-K TTC6 rabbit monoclonal antibody 1 mg x up to 3 Abs 194600 ABN-H00115669-M H00115669-M TTC6 mouse monoclonal antibody (hybridoma) Up to 5 Clones 49000 ABN-H00115669-P01-10ug H00115669-P01 TTC6 (Human) Recombinant Protein (P01) 10 ug 4466 ...
(BMG Labtech, UK). Unstimulated cells were used as negative control. Recombinant murine cytokines also tested included 0.01–10 ng/mL IL-10 (Peprotech, UK), 20 ng/mL IL-1β (Shenandoah Biotechnology, USA), 20 ng/ml Pam3CSK4(Sigma), 50–100 ng/mL TNF (PeproTech), 20...
. We injected MM33 tumor cells into 10 NOG mice and monitored tumor growth by caliper measurement. When the cells had formed palpable tumors, we injected 20×106autologous MM33 TILs per mouse into five mice via the tail vein and treated the mice with recombinant human IL-2, akin to the ...
culturing freshly transducedEpor−/−progenitors for 15 h in SCF and IL3 before transitioning the cells to an Epo-containing medium for the remainder of differentiation. Since SCF and IL3 also promote the growth of myeloid cells, all analysis was performed on cells that were both negative...
Protein lysates were resolved using a 4–16% gradient of polyacrylamide gels and transferred to nitrocellulose membranes (Sigma, St. Louis, MO, USA). The following primary antibodies and dilutions were used: myosin heavy chain (1:1000; R&D Systems, Minneapolis, MN, USA), phospho-p65S536 (1...
6. Invitrogen试剂,部分现货,周三定货,2-3周到货。 7. ABCAM试剂,周三定货,2-3周到货。 8. eBioscience试剂,部分常用现货,周三定货,2-3周到货。 9. STANT CRUZ试剂,周三定货,2-3周到货。 10. Biolegend试剂,周三定货,2-3周到货。 11. Peprotech试剂,部分常用现货,周三定货,2-3周到货。
Recombinant human NOG was obtained from PeproTech. Primocin was obtained from InvivoGen. Prim® hMSC serum-free medium (SF-M) was purchased from Premedical Laboratories Co. Ltd. MSCs chondrogenic differentiation medium was obtained from BGsciences. The RNAeasy™ RNA isolation kit, the ...