Mouse Granzyme B Positive Control OTHER REAGENTS REQUIRED Pipettes and pipette tips Deionized or distilled water Squirt bottle, manifold dispenser, or automated microplate washer 500 mL graduated cylinder 37 °C CO2 incubator Sterile culture media Dissection microscope or an automated ELISpot reader Produ...
The Mouse TNF-α ELISpot assay is designed for the detection of mouse TNF-α secreting cells at the single cell level, and it can be used to quantitate the frequency of mouse TNF-α secreting cells. ELISpot assays are well suited for monitoring cytokine responses to various stimuli, ...
We created a humanized (THX) mouse by grafting NBSGW12and NSGW41 (ref.11) neonates with cord blood huCD34+cells through intracardiac injection, followed by conditioning with 17β-estradiol (E2), the most potent and physiologically abundant estrogen. E2 supports differentiation of HSCs13,14,15,...
ELISpot Plus: Mouse IL-17A (ALP) PRODUCT CODE: 3521-4APW-10 CONTENTS: 10 pre-coated plates, mAb IL17-I Vial 1 (red top) Detection mAb: MT2270, biotin (80 μl) Concentration 0.5 mg/ml Vial 2 (white top) Streptavidin-ALP (150 μl) BCIP/NBT-plus substrate (120 ml) The detection...
IFN-γ Elispot responses by splenocytes were evaluated following immunization with Ebola Zaire GP1,2 peptides. Mice were immunized and boosted 7 days after initial immunization. Splenocytes were re-stimulated in vitro with peptide overnight. The mean IFN- γ Elispots/106 splenocytes +/−...
Immune cell population and distribution can be examined by flow cytometry, immunohistochemistry, and cytokine production by flow, ELISA, or ELISPOT for more robust evaluation of drug properties. Baseline immunophenotypes for PDX tumor-bearing huNSG are often supplied using a 14-color TIL panel. Summa...
Control: Mouse Ig isotype control mixture for generating positive controls 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Substrate: 1X TMB Solution ...
the peptide-stimulated positive controls. with H&E or Black Gold II. Some mouse brain and spinal cord tissues were paraffin-embedded after fixation and stained with H&E or luxol fast blue. The sections were imaged under a light microscope. ELISPOT assays for IFN, IL-17A, IL-10. tai...
ELISPOT assay was carried out using the mouse IFN-γ precoated ELISPOT Kit (Dakewe, Shenzhen, China) according to the manufacturer’s instructions. Briefly, 96-well flat-bottomed microtiter plates were preincubated with the coating antibody (anti-IFN-γ monoclonal antibody) at 4°C overnight and...
Kit Contents for Mouse IFN-gamma ELISpot Kit Mouse IFN-gamma Microplate Biotinylated Detection Antibody Streptavidin conjugated to Alkaline Phosphatase Dilution Buffers Wash Buffer Concentrate BCIP/NBT Chromogen Mouse IFN-gamma Positive Control Other Reagents Required ...