7), samples were mounted and imaged within 30 min after dissection. For LysoTracker staining combined with immunohistochemistry (Fig. 1f), the samples were fixed in 4% formaldehyde for 3 min at room temperature and then stained with antibodies. LysoTracker signals in fat body tissues ...
Multilayered genetic and omics dissection of mitochondrial activity in a mouse reference population. Cell 158, 1415–1430 (2014). CAS PubMed PubMed Central Google Scholar Houtkooper, R. H. et al. Mitonuclear protein imbalance as a conserved longevity mechanism. Nature 497, 451–457 (2013)....
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Notably, coronary, pulmonary, and neurological concerns have been observed in MFS patients and may correlate with findings described in this report. For example, complaints of mitral valve prolapse and regurgitation, cardiomyopathy, coronary artery disease including spontaneous coronary artery dissection, ...
Although the promise of these efforts in contributing to the genetic dissection of complex biological processes is extraordinary, having the appropriate model to which one can apply these emerging technologies is paramount to a successful and productive outcome. Although the mouse has many advantages, ...
Yalcin B, Willis-Owen SA, Fullerton J, Meesaq A, Deacon RM, Rawlins JN, Copley RR, Morris AP, Flint J, Mott R: Genetic dissection of a behavioral quantitative trait locus shows that Rgs2 modulates anxiety in mice. Nat Genet. 2004, 36: 1197-1202. 10.1038/ng1450. Article PubMed CAS ...
(A)RNAs from the mouse Y chromosome was first reported by Bishop and Hatat using a multicopy Y-derived probe, pY353/B [2,35]. Subsequently, more transcripts were identified from repeat sequences localizing to the Y chromosome, in mouse testis [9,10,19,36]. The report ofPirmyandPirmy...
Following fixation, cornea/iris tissue was removed surgically and the retina was isolated carefully under a dissection microscope. The retina was washed three times with PBS in a 48-well plate and permeabilized and blocked simultaneously using 10% normal goat serum with 0.5% Triton X-100 over...
BRAFV600E/PIK3CAH1047R double -mutant Mice, two months after tumor induction, were treated with either a MEK inhibitor (PD-325901) and a class I Pi3'K inhibitor (GDC-0941) for ten days prior to dissection and LAT1 transcription analysis (Fig. 4b). While Pi3'K inhibition did not ...
Plasmacytoid dendritic cells (pDCs) are the main source of type I interferon (IFN-I) during viral infections. Their other functions are debated, due to a lack of tools to identify and target them in vivo without affecting pDC-like cells and transitional