blood, and an air pouch model of acute inflammation. These data were generated using Assay for Transposase-Accessible Chromatin using sequencing (ATAC-Seq), a method that tests genome-wide chromatin accessibility. Briefly, ATAC-seq allows the analysis of chromatin accessibility by sequencing DNA ...
1b). Based on the percentage of GPI-AP-rescued cells, we concluded that T130N was the severest mutation, R119W and K1051E were milder. We next introduced these missense mutations into mouse embryonic stem (ES) cells by CRISPR–Cas9-mediated knock-in (Supplementary Fig. 2) and generated...
The percentage of tooth formation is 6/6. Right: H&E staining of relative tooth sections showing the tooth-like structures containing dental pulp (dp), dentin (d), enamel space (es), and ameloblast layer (Am). Scale bar corresponds to 500 μm (left) and 200 μm (right). (f) ...
Efferent duct ligation (EDL) was performed to block luminal flow from the testis without affecting blood flow. Cytokeratin 5 (KRT5) labeling showed a time-dependent reduction of the percentage of BCs with intercellular projections from 1 to 5 days after EDL, compared to controls. Double labeling...
During the incubation of murine spleen, lymph node, or bone marrow cells with IL-2 (1000 U/ml) a small percentage of cells became adherent to the surface o... Y Gunji,NL Vujanovic,JC Hiserodt,... - 《Journal of Immunology》 被引量: 226发表: 1989年 ...
Nave-phenotype (quiescent) CD8+ T cells of the blood, spleen and mesenteric lymph nodes were identified on the basis of surface coexpression of CD45RA and CD62L. Relative to age-matched controls, the percentage of nave-phenotype CD8+ T cells was high in energy-restricted groups after 9 d...
As shown in Figure 5C, the infection rate (percentage of infected host cells) in A1ATcorr iHLCs infected with P. berghei sporozoites was about 0.21%, which was significantly lower compared with 0.53% in Huh7 human hepatoma cells (used as a comparator) at 48 hpi. Further development of P...
a, Strain-specific SNPs were used to assign sequencing reads to the paternal and maternal allele (Methods). The standard error and mean of the percentage of paternal reads in each stage (yaxis) are plotted.b,d,e, Line plots (top) indicate the percentage of paternal reads (yaxis) in RNA...
In our analysis, the proportions of cell clusters were similar in the caput, corpus, and cauda epididymis. Consistent with a previous study2, the percentage of principal cells was ~60% in the three epididymal regions (Fig. 3a). Intriguingly, the number of segmental differentially expressed genes...
(bothP<0.001), but no effect in the cortex (Figure6B). There was no significant genotype-by-bregma level interaction in any region. Table2shows the mean (± SEM) of the percentage of BrdU+ cells that were Iba1+ per animal in all regions of the brain for both mutant and control mice...