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Figure 3.Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 647, Can Be Used to Stain Epithelial Cells Caco-2 cells were fixed, permeabilized, and labeled with Anti-Acetylated Alpha-Tubulin (Acetyl K40) Antibody, Clone TEU318 (Catalog #100-0753), followed by Goat Anti-Mouse IgG...
The distribution of different tubulin isoforms in the mouse sperm flagellum was studied using four site-directed antibodies to tubulin: DMlA and DMlB general anti a and p-tubulin, 6-116-1anti-acetylated a-tu- bulin, and GT335 anti-glutamylated a and p-tubulin. Quan- titative immunogold ...
(C) Expression of acetylated tubulin levels in control and STE-exposed oocytes. Proteins from 200 oocytes were loaded for each sample. (D) Relative-intensity results for AC-Tubulin protein expression in the control and STE-treated oocytes. Data are presented as mean percentage (mean ± SD...
(ab6586, Abcam, Cambridge, MA, USA), mouse anti-acetylated tubulin (T7451, Sigma-Aldrich), mouse anti-alpha smooth muscle actin (904601, BioLegend, San Diego, CA, USA), guinea pig anti-synaptopodin (internal N-terminus) (GP94-IN, Progen Biotechnik, Heidelberg, Germany), mouse anti-...
MEFs grown on coverslips and cryosections were fixed in ice-cold methanol, blocked with 1% non-fat milk/PBS and immunostained with mouse anti-acetylated alpha tubulin (Sigma) 1:1000; rabbit anti-gamma tubulin (Abcam) 1:500; rabbit anti-β-catenin (Cell Signaling Technology) 1:200. After ...
(a) Kidney sections of Cd79a-Tsc1 KO mice and controls at postnatal day (P) 9 are costained with anti-acetylated α-tubulin antibodies (α-Ac-Tub, green) and DBA (red), markers for cilia and the distal tubule/collecting duct, respectively. Cilia length in DBA-positive cells is ...
I and J, tamKif3a+/− and tamKif3a−/− axonemes labeled with anti-acetylated α-tubulin (Ac-tub) antibody (red) at 1 week (I) and 2 weeks (J) PTI. Relative to tamKif3a+/− photoreceptors (left), Ac-tub-labeled axonemes (red) of tamKif3a−/− photoreceptor OS are ...
Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosp...
(1:1,000; BioLegend, San Diego, CA); rabbit anti-APP C-terminal (1:2,000; AB5352, Chemicon); monoclonal antibody against synaptophysin (1:1,000; AB8049, Abcam, Cambridge, MA); rabbit anti-GAPDH antiserum (1:5,000; G9545, Sigma); and monoclonal anti-β-tubulin III antiserum (1...