We aimed to transplant iPSC-derived motor neuron progenitor cells (iMNP) as a second combination cell source to increase the motor neuron differentiation rate at the lesion site in a chronic SCI model. Trials of ideal cell types and cell transplantation strategies for chronic SCI are required to...
Fig. 1: Motor neuron gene expression is dynamic during embryonic and early postnatal ages. a Timeline of motor neuron development and functional maturation in mice. b A spinal cord section from an Hb9-GFP transgenic E10 embryo and outline of the procedure used to isolate motor neurons. Immunost...
Consecutive 60s sections of the AN motor output under unstimulated and stimulated conditions were analyzed. The neural activity of AN motor pattern for each recording section was analyzed. After the AN recording the ablated CNS was scanned using a confocal microscope (Zeiss LSM780) to identify the...
In preliminary studies we found a 5-fold increase in PAR-1 mRNA in cervical spinal cords fromwr/wr, compared with wild-type (wt) litter-mates. Our current studies suggested that reactive astrocytosis and motor neuron cell death were causally linked with alterations in thrombin signaling. PAR-1...
Thus, the neuron was labeled as active during the type of swim bout if the resulting P value from the test was lower than 0.01. Detection of forward component and steering neurons Using the method stated above, we extracted all neurons active during each bout type as cells whose spiking ...
The number of sections was, in each case, high enough that the series contained the entire nucleus under investigation in the rostro-caudal direction. First, in the fluorescent mode of the microscope, the motor nuclei were located, guided by the anti-motoneuronal (ChAT) signal. Next, the ...
The establishment of functional neuromuscular circuits relies on precise connections between developing motor axons and target muscles. Motor neurons ...
However, neuron-specific EDN1-knockout mice show elevated sensitivity to pain [100], and EDN1 expression in astrocytes is required for their survival under oxidative stress [101]. Moreover, the hypoxia-inducible EDN1 promotes angiogenesis, reducing the possible effects of hypoxia-related oxidative ...
Significant numbers of genes are also under-expressed in UNC-4 motor neurons relative to other embryonic cells. Transcripts showing ≥ 1.7× fold intensity difference in the unc-4::GFP motor neuron vs Reference data sets were defined using SAM statistics at a False Discovery Rate (FDR) of ≤...
Additionally, dysfunction in axonal transport in mature neurons has been associated with various neurodegenerative disorders, including motor neuron diseases such as amyotrophic lateral sclerosis (ALS), Alzheimer’s disease (AD), and Huntington’s disease (HD) [29, 30]....