To measure the size of the soma of cells, the cresyl violet staining served to draw an ROI using Fiji ImageJ software. The drawn ROIs were used to quantify the DAB staining of the soma of individual cells. To accurately quantify only the intensity of the DAB signal without the interference...
To confirm the involvement of Nrf2/HO-1 activation in the neuroprotective effect of UC-CM, cell viability was first evaluated using Nrf2 nuclear translocation inhibitor (Sova and Saso, 2018) ML385 to incubate with UC-CM in both MTT and crystal violet assay (Fig. 6A and B). The significant...