Motoneurons were plated on poly-ornithine/laminin- treated wells in the presence (or not when mentioned) of a cocktail of neurotrophic factors (0.1 ng/ml glial-derived neurotrophic factor (GDNF), 1 ng/ml brain-derived neurotrophic factor (BDNF) and 10 ng/ml ciliary neurotrophic factor (CNTF)...
(B) Western blot analysis for selected components of the mitochondrial respiratory chain subunits I-V: NDUFB8, SDHB, UQCRC2, COX2, ATP5A, as well as mitochondrial transcription factor A(mtTFA) shows an increase in the relative amounts of these proteins (excluding ATP5A) in 28-day-old MNs...