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For each species and class size, the dry propagules were firstly put on a Petri dish, homogenizing the sample of the class size. To avoid biasing the selection of propagules we used a point sampling method that consisted of choosing the first 30 propagules following a line marked beforehand ...
The invention relates to a tilapia mossambica formula feed with rubber seedcakes and soya bean meal serving as main protein raw materials, and belongs to the technical field of aquaculture. The tilapia mossambica formula feed comprises the following raw materials in percentage by weight: 4 to 8...
The average intensity at each time point was calculated as the intensity of the GFP signal divided by the area of the cell. The movie of the time-lapse images was edited with ImageJ 1.48v. Images of PpCSP1-Citrine localization (Fig. 2c) were taken by an inverted microscope (IX81, ...
Cultures transferred to 12°C and 25°C were sampled every 4th hour for 52 hours, two time points in light followed by 12 time points in DD, as indicated in each figure. RNA sample preparation and cDNA synthesis Total RNA was extracted from each time point sample using the RNeasy plant ...
The news workbench solution was originally conceived during the redesign of the MSW portal from SharePoint Portal Server 2003 to MOSS 2007 - to address the problem of effectively managing the discovery, selection, and distribution of news content published to the site or emailed to subscribers....
“At this point in time, Clare and Dale aren’t getting back together,” a source close to Clare says. “They want to be friends and do want to see each other but don’t think they have a long-term future together. Clare is trying to move on, but she is still in contact with ...
Total RNA was extracted from each time point sample using the RNeasy plant mini kit (Qiagen). For each sample, 0.5 μg of total RNA was reverse transcribed to cDNA using random hexamer primers (Invitrogen) and Superscript III reverse transcriptase (Invitrogen), following the manufacturer's inst...
The past two decades, my colleagues and I have engaged in the study of the functional analyses of the moss, P. patens PPR (PpPPR) proteins with an evolutionary point of view, relative to the PPR proteins in land plants. P. patens (hereafter Physcomitrium) has emerged as a powerful mod...