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We then tested whether, in response to heat, the cytosolic GAPCs might enter the nucleus where they could interact with the transcription factor NF-YC10. Arabidopsis transgenic lines overexpressing GAPC1 or GAPC2 fused with the green fluorescence protein (GAPC-GFP) were generated to observe their...
Then, all crystals were flash-cooled to 100 K in a stream of cold nitrogen prior to data collection. X-ray diffraction data were collected on SPXF beamline BL13C1 at the National Synchrotron Radiation Research Center in Taiwan. The diffraction images were processed using the HKL2000 program ...
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